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Nano sol-gel film electrode, its preparing method and use

A nano-sol, gel film technology, applied in the direction of material analysis, material inspection products, measuring devices, etc. by electromagnetic means, can solve the problems such as no immune sensor reported in literature, low sensitivity, complicated operation process, etc.

Inactive Publication Date: 2006-05-31
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of the above immunoassay methods still has limitations such as the need for optical labeling or enzyme labeling for the detection of target molecules, complex operating procedures, and low sensitivity.
Capacitance immunoassay, as a highly sensitive, label-free immunoassay method, has been successfully applied to immunosensors based on self-assembly technology, but so far there has been no literature report on sol-gel capacitive immunosensors

Method used

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  • Nano sol-gel film electrode, its preparing method and use
  • Nano sol-gel film electrode, its preparing method and use
  • Nano sol-gel film electrode, its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 The influence of water content in the sol-gel system on the thickness of the final gel biofilm

[0035] In the gel system, the water content in the sol will directly affect the thickness of the final gel biofilm. Therefore, the present invention measures Al with different water content. 2 O 3 -Ab, SiO 2 -Ab biofilm thickness, the list is as follows:

[0036] Al 2 O 3 (nm)

[0037] 5.

[0038] The results show that for Al 2 O 3 Sol system, Al:H 2 When O=1:100, the thickness of the biofilm can be about 40nm. By further increasing the water content in the sol to 1:300, the thickness of the biofilm decreased to about 20 nm. In contrast, for SiO 2 Biofilm, usually used to embed biomolecules in SiO 2 Si: H in the sol 2 O is 1:10, and the thickness of the film is 350~370nm, which is similar to the measurement result of the SEM method (Liu ZJ, Liu BH, Kong JL, Deng JQ. Anal. Chem. 2000, 72, 4707). Increasing the water content to 1:100, the thicknes...

Embodiment 2

[0039] Example 2 further reduces Al 2 O 3 Thickness of gel biofilm

[0040] Al 2 O 3 Although the thickness of gel biofilm is much smaller than SiO 2 The system is still thicker than the self-assembly layer, and its final capacitance is greater than the capacitance of the immunosensor constructed based on the self-assembly of sulfhydryl compounds (Table 2). Therefore, the electrode capacitance can be effectively increased when the biofilm is thick, thereby increasing its RC constant. The increase of this value is essential for realizing multi-channel sampling point analysis. Because for the multi-channel potential step, the A / D noise of the instrument will be greater than the single-channel state. In order to ensure the good linearity of the fitted curve, the RC constant of the immunosensor must be increased so that the actual system is closer to the RC model, thereby realizing multi-channel sampling point analysis. Table 4 also lists the constructed immunosensor data, and its fit...

Embodiment 3

[0042] Example 3 The biofilm of the sensor after the long-chain alkyl alcohol is sealed is in an insulating state

[0043] In this experiment, [Fe(CN) 6 ] 3- / 4- It serves as a redox probe to monitor the formation stage of the insulating layer. Using conventional methods to detect figure 1 Shown. figure 1 a is the bare gold electrode in 5mM[Fe(CN) b ] 3- / 4 Cyclic voltammogram in solution. When the sol without antibody is directly dropped on the surface of the electrode, the dried gel film cyclic voltammogram ( figure 1 b) It shows that the current value is smaller than that of the bare gold electrode, indicating that the gel film can be effectively fixed on the electrode surface. The mixed antibody molecules and sol are on the electrode surface, and the electrode current after drying is further reduced, such as figure 1 As shown in c, it is proved that the antibody molecule has been successfully immobilized in the gel system. The shape of the cyclic voltammogram presents a posi...

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Abstract

The invention relates to the field of basic electrical components, and provides a nano sol-gel film electrode, its preparation method and application. Electrochemical immunosensor is a new method for antigen determination. At present, although the preparation of sensors by electrochemical immunoassay breaks through the limitations of common immunoassay methods, such as the need to optically or enzymatically label the detection target molecule, the operation process is complicated, and the sensitivity is not high, but it is often used due to the lack of an appropriate system for immobilizing antibodies. Its application is limited. The invention provides an electrode covered with a nano-sol-gel film. The film covered on the electrode is a nano-sol-gel film, which is sealed with a long-chain alkyl alcohol to insulate it; antibodies are fixed in the gel, and the electrode is measured by The capacitance change detects the content of the substance that can be combined with the antibody in the analyte. The electrode of the invention can be used to make a sensor for detecting biomolecules, and can also be used to make an electrochemical detection instrument capable of measuring the concentrations of multiple compounds at one time.

Description

Technical field [0001] The invention relates to the field of basic electrical components. Specifically, the invention provides an electrode containing a nano ultrathin sol-gel film and a preparation method thereof. The invention also provides the application of the electrode in making electrochemical immunosensors and electrochemical instruments for detecting disease markers. Background technique [0002] Electrochemical immunosensor is a new method of antigen determination based on electrochemical test methods and biological immune recognition reaction. The key step involved is to immobilize antibodies on the electrode surface to form highly active recognition sites. Recently, the self-assembly method of gold electrode surface has shown great potential. Combined with covalent immobilization reaction, an orderly insulating monomolecular antibody insulating film can be constructed, which is suitable for the application of capacitive immunoassay. However, this method uses covalent ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/333G01N33/53
Inventor 孔继烈江德臣刘宝红张松
Owner FUDAN UNIV