Method for purifying composite interferon

Abstract

The invention provides a method for purifying a compound interferon, which comprises the following steps: adding buffer D to the compound interferon obtained by denaturing the inclusion bodies fermented and collected by conventional methods, adding buffer D while stirring, and then adding the compound interferon that can permeate the molecular weight below 50000 In the dialysis bag, dialyze in the buffer E to carry out the renaturation of the complex interferon protein; separate the supernatant, and use buffer F and buffer G gradient elution on the chromatographic column equipped with strong cation exchange medium, Obtain purified compound interferon. The method can not only completely remove impurity proteins with a molecular weight of 30,000 to 40,000, but also basically separate the refolded intermediates from the fully refolded proteins, so that the obtained compound interferon has high purity and a more uniform product structure.

Description

technical field

[0001] The invention relates to the field of biotechnology, and relates to a preparation process of recombinant composite interferon (Consensus interferon, IFN-α-conl), in particular to a method for one-step purification of recombinant composite interferon by using a cation exchange medium. Background technique

[0002] Interferon (IFN, Interferon) is a general term for a class of cytokines with broad-spectrum anti-virus, anti-proliferation and immune regulation, which can activate natural killer cells (NK cells) and other functions. It is a low-molecular-weight glycoprotein with similar structure, similar function and biological activity produced by cells induced by various inducers, and is an important class of cytokines. Interferons are generally divided into type I and type II. Type I includes IFN-α, IFN-β and IFN-ω, and IFN-α can be divided into more than 25 subtypes with different primary structures; type II includes IFN- γ . These interferons are enc...

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