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Calf blood deproteinizing extract, its eye ointment preparation and its preparing method

A protein-removing and extracting technology, which is applied in its preparation method and eye ointment preparation, and calf blood protein-removing extract field, can solve the problem that there is no manufacturer, calf blood is produced, and it is difficult to buy imported products of bovine origin and other issues, to achieve the effect of drug safety, significant curative effect, and guaranteed effectiveness

Active Publication Date: 2006-11-29
ZHAOKE PHARMA HEFEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, several companies in China are producing calf blood protein-free extract injection, but there is no manufacturer producing calf blood protein-free extract eye ointment
Moreover, due to the impact of mad cow disease in Europe, my country has restricted the import of bovine-derived drugs, and it is difficult to buy imported bovine-derived products (including Sugaojie Eye Ointment) on the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of calf blood deproteinized extract

[0034] (1) Preparation of red blood cells: Take a stainless steel bucket, add 10L of 3.8% sodium citrate in advance as an anticoagulant, collect 100L of fresh calf blood from 1 to 6 months old under sterile conditions, and stir slowly to prevent blood coagulation . The collected blood was centrifuged at 3000 rpm for 20 minutes, and the supernatant was poured off gently. Add an equal amount of normal saline, mix well, centrifuge at 3000 rpm for 15 minutes, discard the supernatant, wash 3 times in the same way, and collect 40L of precipitated red blood cells.

[0035] (2) Broken red blood cells: Add 200 L of deionized water to the red blood cells at a ratio of 1:5, stir evenly, and place in a -20°C refrigerator for quick freezing overnight. Take it out the next day, and heat it up rapidly in a 40°C water bath to melt, destroy the red blood cell membrane, release the contents, and obtain a total of 240L of solut...

Embodiment 2

[0042] Example 2 Preparation of calf blood deproteinized extract eye ointment

[0043] (1) prepare raw materials by the formula shown in table 1;

[0044] Element

Percentage (%)

mass (g)

Calf blood deproteinized extract

30

1500

Sodium carboxymethyl cellulose

1.2

60

Glycerin

5

250

Ethylparaben

0.1

5

Deionized water

Add water to a total weight of 5000g

[0045] (2) Preparation of eye ointment base: Take a 10L container, weigh 250g of glycerin, add 60g of sodium carboxymethylcellulose, stir with a glass rod, disperse evenly, add 5g of ethylparaben, add about 3000g of deionized water, Stir evenly, place in an autoclave, sterilize at 121°C for 20 minutes, take out, cool, and set aside.

[0046] (3) Add raw materials: Add 1500g of deproteinized calf blood extract to the matrix under aseptic conditions, add deionized water to a total weight of 5000g, stir evenly with a ste...

Embodiment 3

[0047] Example 3 Study on the biological activity and quality of calf blood deproteinized extract eye ointment

[0048] (1) Biological activity measurement: This product is an activator of cellular respiration, which can promote the uptake and utilization of oxygen by cells. The activity of promoting cellular respiration in guinea pig hepatocytes was measured by WARBURG micro-respiration manometer, and its biological activity was calculated from the increase of oxygen consumption. The biological activity of three batches of samples of calf blood deproteinized extract eye ointment was determined, and the results are shown in Table 2.

[0049] batch number

20011027

20011028

20011029

QO 2 (ul·O 2 / mg h)

6.32

6.30

6.35

Si

5.44

5.43

5.47

[0050] (2) Quality research: See Table 3 for the quality data comparison results between this product and Sugaojie Eye Ointment.

[0051] sample

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PUM

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Abstract

The present invention discloses calf blood deproteinizing extract, its eye ointment preparation and the preparation process. The preparation process of calf blood deproteinizing extract includes the following steps: collecting calf blood, separating erythrocyte, adding water to swell to destroy erythrocyte membrane and to release inside matter, pasteurization, ultrafiltering and concentration to obtain the intermediate; nano filtering to desalt, reverse penetration to concentrate and decolorizing to obtain calf blood deproteinizing extract. Adding proper amount of excipient and stabilizer into the calf blood deproteinizing extract obtains calf blood deproteinizing extract eye ointment preparation. The said preparation process has less loss of effective components and can maintain the bioactivity at most. The eye ointment preparation is used in treating keratitis, cornea ulcer and other eye diseases via promoting the restoring and regeneration of tissue.

Description

technical field [0001] The invention relates to the field of animal blood products, and more specifically relates to calf blood deproteinized extract, its preparation method and eye ointment preparation. Background technique [0002] The "protein-free calf bloodextract" extracted from calf blood is a small molecule biologically active substance, containing small molecule polypeptides, amino acids, nucleotides, keto acids and other substances. Its main pharmacological effect is to enhance the uptake and utilization of oxygen and glucose by cells, promote the synthesis of ATP, promote the delivery of nutrients, and promote the recovery and regeneration of tissues. It can be used to treat patients with cerebral metabolic disorders and cerebral insufficiency, and can also be widely used to treat corneal ulcers, skin ulcers and injuries, bedsores, cosmetic surgery, [0003] Repair and regeneration after plastic and orthopedic surgery have a very wide range of clinical applicatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/14A61K9/06A61P27/02
Inventor 杨中强张国辉李兆羿
Owner ZHAOKE PHARMA HEFEI
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