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Polyepitope hepatitis C antigen complex polypeptide vaccine and its prepn and application

A hepatitis C and polypeptide vaccine technology, applied in the field of molecular biology, can solve problems such as the inability to find a breakthrough point in the development of hepatitis C vaccine

Inactive Publication Date: 2002-05-08
楚雄老拨云堂药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, in the above-mentioned research all can not find the breakthrough point of developing hepatitis C vaccine

Method used

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  • Polyepitope hepatitis C antigen complex polypeptide vaccine and its prepn and application
  • Polyepitope hepatitis C antigen complex polypeptide vaccine and its prepn and application
  • Polyepitope hepatitis C antigen complex polypeptide vaccine and its prepn and application

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Experimental program
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Effect test

preparation example Construction

[0073] ⑤ Preparation of polypeptide immunogen:

[0074] Measure the effective protein concentration of the above-mentioned purified HCV multi-epitope antigen complex polypeptide by ultraviolet spectrophotometer, prepare 5 μg, 10 μg, 20 μg / 100 μl concentration solution according to the actual concentration, add formaldehyde to 1 mg / ml, 37 ° C for 1 hour, Add Al(OH) 3 To 0.5mg / ml, shake at room temperature for 3 hours, then it can be used for immunization of experimental animals.

experiment example

[0076] 1. The immunoprototype and antigenic characteristics of HCV multi-epitope antigen complex polypeptide.

[0077] Specific antibodies can be detected after immunizing mice with the HCV multi-epitope antigen complex polypeptide three times. Experimental analysis shows that the complex polypeptide can induce the production of HCV antibodies in animals. The antibody titers after ELISA detection are shown in Table 1 . On this basis, the immune effects of various adjuvants and different doses were explored, see Table 2 for details. Table 1, complete Freund's adjuvant and HCV multi-epitope antigen complex polypeptide immunized mice with serum antibody titer 3 times

[0078] Group n antibody titer

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Abstract

The present invention relates to molecular biological technology and aims at providing one kind of immunogenic hepatitis C vaccine to HCV virus with serious harm. The said polyepitope antigen complexpolypeptide consists of 131 amino acids and has molecular weight of 13.8 KD and isopotential point of 8.3. Its preparation includes the steps of gene design, effective expression, separation and purification of expression product and preparation of polypeptide immunogen. Test data shows that the vaccine of the present invention has excellent scene of being used as hepatitis C preventing vaccine and detection and diagnosis material.

Description

1. Technical field [0001] The invention relates to the technical field of molecular biology, in particular to a vaccine prepared by using organisms. 2. Background technology [0002] Viral hepatitis is a serious disease that endangers human health. Its pathogen is a class of hepatropic viruses with different structures. So far, there are 7 types of hepatitis viruses that have been discovered, and they are HAV, HBV, and HCV. , HDV, HEV and possibly TTV and HGV. The hepatitis caused by these hepatitis viruses all shows the necrosis of liver parenchymal cells, which has a serious pathological effect on the function of the liver, thus causing great harm to the human body. Since no effective antiviral drugs have been found so far, the prevention and treatment of various types of viral hepatitis focuses on the prevention of vaccines. With the advancement of biotechnology, the application of live attenuated hepatitis A vaccine and inactivated vaccine and hepatitis B genetically e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/29C12N15/51
Inventor 李琦涵王烔董承红王丽春赵红玲赵树栋孙明胡方刘龙丁董少忠马绍辉王晶晶和绍清
Owner 楚雄老拨云堂药业有限公司
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