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Microbe(No.4) and production thereof

A technology of microorganisms and culture medium, applied in the field of microorganisms, can solve the problems of no effective tobacco leaf alcoholizing agent, etc., and achieve the effects of fine aroma, harmonious smoke, and improved aftertaste

Inactive Publication Date: 2003-09-03
王革
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of microorganism that can be used to improve the quality of finished tobacco and the availability of upper tobacco leaves and low-grade tobacco leaves and its preparation method

Method used

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Experimental program
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Embodiment Construction

[0014] specific implementation plan

[0015] The microorganism of the present invention is Bacillus mycoides No. 4 isolated from tobacco leaves, and its preservation registration number is CGMCC No. 0676.

[0016] The cultivation method is as follows: first adopt solid bean sprouts juice medium YBA, inoculate the above-mentioned bacteria on the slant medium of the test tube, activate and cultivate at 27-29°C, and then use fluid bean sprouts juice medium YB to inoculate the mushrooms in the test tube two days later. Bacillus spp. was inoculated in Erlenmeyer flask YB bean sprout juice fluid medium and cultured on a herbal shaker at 28°C for 24 hours.

[0017] Its liquid fermentation preparation method is carried out according to the following steps:

[0018] 1) Test tube culture medium: use sterilized solid bean sprout juice (YBA) medium to inoculate Bacillus mycoides on the test tube medium, and activate and cultivate at 27-29°C for 48 hours after inoculation;

[0019] 2) Ex...

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PUM

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Abstract

A Bacillus mycoides No.4 (CGMCC No.0676) is prepared by separating it from tobacco leaf. It can decrease the irritation of cigarette smoke and the protein and nicotin of cigarette, and improve the smell and taste of cigarette.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a microorganism (No.4) and a preparation method thereof. Background technique [0002] At present, when raw tobacco and green tobacco that have not been naturally fermented are mixed into industrial formulas, they bring great irritation to the smoke, pungent taste, rough, astringent mouth, stagnant tongue, insufficient or lack of aroma, and burning Bad sex and other shortcomings. This not only brings greater damage to the inner quality, product style and other aspects of finished cigarettes. Furthermore, at present, nearly 60% of the top tobacco leaves and low-grade tobacco leaves of major tobacco factories and tobacco companies in the country have a large backlog due to low utilization rate, occupying a large amount of warehouses and funds. Contents of the invention [0003] The object of the present invention is to provide a microorganism and its preparation method w...

Claims

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Application Information

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IPC IPC(8): A24B15/20C12N1/20
Inventor 王革
Owner 王革
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