Acellular short corynebacteria preparation

Corynebacterium brevis, cell-free preparation technology, applied in the field of immunomodulators and cell-free Corynebacterium brevis preparations, can solve problems such as cell fragmentation, achieve uniform particle size, improve drug efficacy, and reduce fever.

Inactive Publication Date: 2004-01-07
上海昌润生物科技有限公司
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many methods for cell disruption, it is di...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Acellular short corynebacteria preparation
  • Acellular short corynebacteria preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] According to the method described in the 2000 edition of "China Biological Products Regulations", the establishment, verification and other verification of bacterial seed batches were carried out.

[0055] The medium used in this example is the thioglycolate medium (without agar) produced by Beijing Sanyao Science and Technology Development Company, and its ingredients are: tryptone 15g / l, yeast extract powder 5g / l, glucose 5g / l , sodium chloride 2.5g / l, L-cystine 0.5g / l, sodium thioglycolate 0.5g / l. The medium was filtered through a 0.22 μm membrane before use.

[0056] Open the CP (Propionibacterium acnes, scientific name, propionibacterium acnes) 771 (see "China Biological Products Regulations") strain tube, each tube contains 6 billion bacteria, and draw about 0.2-0.3ml of thioglycolate medium (not containing agar), add it to the bottom of the strain tube to dissolve it completely, suck it out, add it to the middle tube (capacity 38ml), mix it with 8-10ml of the ab...

Embodiment 2

[0061] Referring to the method of Example 1, CP (scientific name propionibacterium acnes, propionibacterium acnes) 65101 (76-27) was inoculated in a nutrient tank after 4 consecutive passages, and was cultured in a thioglycolate medium (without agar) (before use) Filter the culture medium with a 0.45 μm membrane) and culture at 36-37°C for 6 days; check the bottles one by one under the microscope, and discard those contaminated by bacteria; combine and collect the bacteria without bacteria contamination, heat and boil for 60 minutes, and use ultra-high pressure jet Collider at 1500kgf / cm 2 Broken bacterium under pressure 5 times; The broken suspension was centrifuged at 8000rpm for 1 hour, washed with sterile normal saline for 5 times, sterility test (2000 edition "China Biological Products Regulation") and pyrogen detection (2000 edition Pharmacopoeia of the People's Republic of China ") is qualified to make a suspension with a solid content of 2.0mg / ml, and heat at 65°C for ...

Embodiment 3

[0065] With reference to the method of Example 1, CP (Propionibacterium acnes) 65102 (H-84) was inoculated in a nutrient tank after 4 consecutive passages, and cultured in a peptone medium at 36-37°C for 7 days; bottle by bottle Microscopic examination, those with contamination by miscellaneous bacteria were discarded; combined and collected bacterial cells without contamination by miscellaneous bacteria were heated and boiled for 30 minutes. 2 Broken thalli under pressure 10 times; The suspension after broken is centrifuged at 12000rpm, washes precipitate 4 times with sterile normal saline, sterility test (2000 version "Chinese Biological Products Regulations") and pyrogen detection (2000 version "People's Republic of China Regulations") Pharmacopoeia ") qualified to make a suspension with a solid content of 1.0mg / ml, heated at 65°C for 100 minutes to obtain NCP.

[0066] Detect (solid content 1mg / ml) NCP pyrogen 60EU / ml by limulus reagent method (2000 edition "Pharmacopoeia ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Granularityaaaaaaaaaa
Granularityaaaaaaaaaa
Granularityaaaaaaaaaa
Login to view more

Abstract

The present invention discloses a non-cell corynebacterium preparation (NCP). It is a non-cell preparation prepared from corynebacterium parvum (CP), its heat source is less than 160 EU/ml, protein content is 10-40 % (g/g), nucleic acid content is 3-25% (g/g), and the rest is polysaccharide, and its grain size is less than 100 nm. Said invented NCP is a nano preparation made up by breaking CP to nano-grade, and is a good immune regulating agent.

Description

technical field [0001] The invention relates to an immunomodulator, in particular to a cell-free short coryneform bacterium preparation (NCP). The NCP is a cell-free preparation prepared by crushing a short coryneform bacterium (CP), belonging to the field of biological products. Background technique [0002] Corynebacterium breve bacterin (CPV, 1995 edition of "China Biological Products Regulations") or short Corynebacterium preparations (CPP, 2000 edition of "China Biological Products Regulations") is a non-specific immunomodulator. It is a dead bacterin vaccine made from Corynebacterium brevis (CP) inactivated by formaldehyde, and its preparation process is disclosed in the 1995 and 2000 editions of "China Biological Products Regulations". Currently used CPP preparations also include inactivated vaccines produced by Merleux Research Institute in France; inactivated vaccines produced by Wellcome Pharmaceuticals in the UK, etc. [0003] CPP has the ability to activate the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/74A61P37/02
Inventor 高尚先李守悌
Owner 上海昌润生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap