Preparation method of nano titanium dioxide-bio-protein compasite membrane electrode and its application

A technology of nano-titanium dioxide and biological protein, which is applied in the field of materials and bioengineering, can solve the problems of difficult to ensure the uniformity and smoothness of the film, and is not suitable for the fixation and modification of light-sensitive proteins, so as to improve the photoelectric conversion efficiency, broaden the absorption wavelength range, The effect of increasing sensitivity

Inactive Publication Date: 2004-02-25
FUDAN UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the titania sol-gel reported in the patent is acidic in pH value, while light-sensitive proteins usually have the best photoelectric conversion efficiency in a neutral or even slightly alkaline environment; and the electrode area is larger It is difficult to ensure the uniformity and smoothness of the film
Therefore, this method is not suitable for immobilization and modification of light-sensitive proteins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Measure 15% TiCl 3 Solution 5ml, add about 20ml pure water, pass N 2 Deoxygenation for about 20 minutes. Slowly add 10% K under stirring, dark and nitrogen 2 CO 3 Solution until the pH value is about 2, slowly add cetyltrimethylammonium bromide about 0.046g under the above conditions, continue to use K 2 CO 3 The pH of the solution was adjusted to 2.40. Formulated TiCl 3 cetyltrimethylammonium bromide and TiCl in solution 3 The molar ratio is close to 5%. With the above TiCl 3 The solution is an electrolyte, and eight pieces of ITO conductive glass with the same area, about 1.5cm×2.5cm, are working electrodes, platinum sheets are auxiliary electrodes, and saturated calomel is a reference electrode. In a static state, the control potential is 0.1V. Electrodeposition of TiO for 40min 2 , the whole process needs to avoid light and deoxygenation. After the reaction is stopped, the obtained TiO 2 After the membrane was washed with pure water and dried in an oven,...

Embodiment 2

[0023] Measure 15% TiCl 3 Solution 5ml, add about 20ml pure water, pass N 2 Deoxygenation for about 20 minutes. Slowly add 0.5M KOH solution dropwise under stirring, avoiding light and blowing nitrogen until the pH value is about 2, slowly add about 0.069g of cetyltrimethylammonium bromide under the above conditions, and continue to adjust the pH with KOH solution value to 2.40. Formulated TiCl 3 cetyltrimethylammonium bromide and TiCl in solution 3 The molar ratio is close to 8%. In the self-made eight-channel electrolytic cell, with the above TiCl 3 The solution is an electrolyte, and eight pieces of the same area, about 1.5cm×2.5cm of conductive glass (ITO) are used as working electrodes, platinum sheets are used as auxiliary electrodes, and saturated calomel is used as reference electrodes. In a static state, the control potential is 0.15V , the time is 30min to electrodeposit TiO 2 , the whole process needs to avoid light and deoxygenation. After the reaction is s...

Embodiment 3

[0025] The preparation and treatment process of titanium dioxide are the same as in Example 2. in TiO 2 After the film was baked and cooled to close to room temperature, it was placed in pH 8.0 Tris with a concentration of 1.2 mM of purple bacteria light and reaction center protein RC (extracted from the wild strain R26 of purple bacteria Rb.Sphaeroides, provided by Shanghai Institute of Plant Physiology, Chinese Academy of Sciences). -HCl buffer. Nano-titanium dioxide-bacteria light and reaction center protein composite membrane electrodes were obtained after adsorption in a refrigerator at 4°C for 3 days. In the Tris-HCl solution of 8mM sodium dithionite, with a 60W white weaving lamp as the light source, the measured short-circuit photocurrent of the nano-titanium dioxide-bacteria light and reaction center protein composite membrane electrode is about 2.8 microamps, which is the same condition The short-circuit photocurrent (1.2 microamps) of the nano-titanium dioxide ele...

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Abstract

The present invention adopts electric deposition process to prepare nano TiO2 porous film electrode firstly, then uses said electrode to adsorb and fix bioprotein so as to obtain the invented nano titanium dioxide-bioprotein composite film electrode with good transmittance, uniformity and stability. The composite film electrode can be used for making the devices of solar cell and light-operated switch, etc. and can raise its sensitivity and photoelectric conversion efficiency.

Description

technical field [0001] The invention belongs to the field of materials and bioengineering, and relates to a method for manufacturing a nano-titanium dioxide-biological protein composite membrane electrode and an application of the method. Background technique [0002] Nano-titanium dioxide has become a widely studied nano-semiconductor material because of its specific properties and excellent performance that cannot be compared with bulk materials. It has good application prospects in the fields of photoelectric conversion, electrochromism, and photocatalysis. The photoelectrode made of nano titanium dioxide porous film has high photoelectric conversion efficiency, high working current density and good stability, and is often used in the research and development of solar cells. The nano-titanium dioxide porous membrane electrode can not only be used as an effective carrier for immobilizing biological photosensitive proteins, but also can be sensitized by coupling with the ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/26G01N27/327
Inventor 孔继烈陆一东刘宝红张松
Owner FUDAN UNIV
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