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Preparation method for producing fibrin gel and platelect gel using human thromblase

A fibrin glue, thrombin technology, applied in blood diseases, extracellular fluid diseases, skin diseases, etc., can solve problems such as antigenic doubts, pathogenic protein particles, immune reaction bleeding, etc.

Inactive Publication Date: 2004-06-30
新扬行实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] However, due to the lack of thrombin made from human plasma, the latter method requires the use of thrombin derived from fetal bovine serum, which is a heterologous protein, and there are various antigenic concerns and pathogenic protein particles ( prion) infection, and even cause severe immune reactions and fatal bleeding, because some patients will develop anti-FV antibody, which cross-reacts with its own fifth clotting factor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] In the preparation method of the present invention for producing fibrin glue and platelet gel with human thrombin, the steps and optimal conditions used in the preparation of thrombin are determined by the following experimental operations:

[0022] 1. Calcium chloride (CaCl 2 ) Determination of concentration:

[0023] Add the calcium chloride solution with three evaluated final concentrations of 20mM, 50mM, and 80mM to the plasma containing glass beads, activate the plasma under several stages of time control, and then mix the activated plasma with the frozen precipitate and measure The results of the reaction time to produce fibrin glue are shown in Table 1.

[0024] 20mM (10 minutes)

50mM (10 minutes)

80mM (10 minutes)

Formation time

30-40 seconds

Not formed

Not formed

20mM (20 minutes)

50mM (20 minutes)

80mM (20 minutes)

Formation time

30-40 seconds

>4 minutes

Not formed

20mM (30 minutes)...

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Abstract

The present invention relates to a method for producing fibrin colloid and preparing platelet gel by using human thrombosin, in particular it utilizes ingle blood donor or self-body thrombin to produce fibrin colloid and platelet gel with bio-compatibility and bio-degradability. Said invented preparation method can reduce viral infection and antigenic hazard, and can raise application of fibrin colloid in the fields of medicine and cell culture.

Description

(1) Technical field [0001] The invention relates to a preparation method of fibrin glue and platelet gel, in particular to a preparation method of fibrin glue and platelet gel produced by human thrombin. (2) Background technology [0002] Fibrin glue, also known as Fibrin sealant or Fibrin adhesive, can be achieved by mixing two plasma components-Fibrinogen concentrate and coagulation. Enzyme concentrate (Thrombin concentrate) has become one of the most important products of human plasma. [0003] Fibrin glue is a plasma product used locally. It is the last step of simulating the blood coagulation mechanism and finally forms a fibrin clot to adhere to the local tissue to which it is applied. Fibrin glue helps to stop bleeding and wound healing. Therefore, its application is mainly in medical operations, such as wound healing, orthopedic surgery, skin transplantation, bone reconstruction or burn and scald treatment. It can be used in cell culture. It becomes the culture substrate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/16A61L24/10A61P7/04A61P17/02
Inventor 泰瑞・玻诺夫
Owner 新扬行实业有限公司
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