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Fast detection device and method for HIV antibody in urine

A technology of antibody and target antibody, which is applied in the field of immunochemistry and biochemical analysis, and can solve problems such as unsolved rapid detection methods

Inactive Publication Date: 2004-12-29
BEIJING KELIPUTE BIOPHARML TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In response to these problems, sandwich methods and sensitive detection methods using metal sols or other colored particles have been developed, however, these methods do not solve all the problems encountered in rapid detection methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Example 1 Evaluation of Selectivity and Sensitivity of Immunoassay Devices to Frozen and Fresh Samples

[0091] This example shows that the immunoassay device manufactured according to the present invention has very high sensitivity and specificity for AIDS samples from different sources.

[0092] The immunoassay device of the present invention comprises the following components:

[0093] Fiberglass sample area pads were blocked and loaded with a solution containing polyvinylpyrrolidone, bovine serum albumin, avian serum, carbonate buffer (0.5M) and Triton-X 100. Squeeze the sample pad to remove excess liquid and dry overnight at 30°C.

[0094] The pad for the assay area included HIV antigen crosslinked to a polyester nonwoven membrane using a streptavidin / biotin crosslinker. Briefly, a 100 mg / ml avidin solution and a 10 mg / ml HIV-1 polypeptide and HIV-2 polypeptide solutions were prepared. The avidin solution and the HIV solution were mixed according to the ratio of...

Embodiment 2

[0101] Embodiment 2 rapid immunochromatography (RIC) one-step detection result

[0102] This example illustrates that the cassette assay method is suitable for detecting HIV antibodies in urine. Although the antibody levels in urine were lower than in blood or serum, the method was successfully detected in urine confirmed by EIA and Western Blot. Rapid immunochromatography (RIC) is used to detect HIV antibodies in blood / serum, which plays an important role in reducing the rate of HIV transmission. The studies described below used the HIV-1 RIC assay for its potential ability to detect HIV-1 antibodies in urine.

[0103] METHODS: Evaluation of the rapid test was performed by testing 11 cryopreserved archived urine samples, detected by the Calypte HIV-1 urine EIA method, confirmed by the Cambridge Biotech HIV-1 urine Western Blot method, 6 out of 11 urine samples One was positive for anti-HIV-1 antibody, and four were negative for anti-HIV-1 antibody. Urine samples were mixed...

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PUM

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Abstract

The present invention provides device and method for fast detection of endogenous antibody, especially HIV envelope protein resisting antibody, in urine. Other pathogens, including known pathogen of sexually transmitted disease, capable of causing the raising of endogenous antibody may be also detected with the device and method of the present invention.

Description

technical field [0001] The invention relates to immunochemical and biochemical analysis methods, and provides a device and method for rapid detection of urine endogenous antibodies (especially specific antibodies against HIV virus envelope protein). Other pathogens that can cause the rise of urinary endogenous antibodies, including currently known pathogens of sexually transmitted diseases, can also be detected by the present invention. Background technique [0002] A number of lateral flow assays have been used to detect various substances in body fluids such as blood or urine. These detection methods include: antigen-antibody reactions, synthetics cross-linked with enzymes, fluorescent substances or visual markers, and specially designed reaction chambers. In most of these assays, there is a receptor (such as an antibody) specific for a particular antigen, and a set of means to detect the presence and / or amount of the antigen-antibody complex. This test design is mostly ...

Claims

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Application Information

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IPC IPC(8): G01N33/53
Inventor 罗那多·明克托比·告特弗瑞德约翰·安尼斯保罗·斯告特
Owner BEIJING KELIPUTE BIOPHARML TECH
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