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Combined reagent in colloidal gold method for detecting syphilis antibody and hepatitis b surface antigen

A technology of hepatitis B surface antigen and combined detection, which is applied in measurement devices, instruments, scientific instruments and other directions, can solve the problem of lack of combined detection reagents, etc., and achieve the effects of convenient, fast and accurate detection methods and simple and clear experimental results.

Inactive Publication Date: 2005-06-15
深圳益生堂生物企业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing commercialized reagents detect syphilis or hepatitis B surface antigen separately, but there is no report of combined detection reagents

Method used

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  • Combined reagent in colloidal gold method for detecting syphilis antibody and hepatitis b surface antigen
  • Combined reagent in colloidal gold method for detecting syphilis antibody and hepatitis b surface antigen

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specific Embodiment approach

[0009] DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT The product is exclusively produced by the applicant unit of the present invention

[0010] preparation procedure

[0011] 1 Preparation of detection reagent strips

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Abstract

This method can test if a patient has been infected by syphilis or hepatitis B virus with a drop of blood, which combines two test measures for testing diseases of syphilis antibody and hepatitis B surface antigen on a same test paper, utilizes immune chromatographic technology and automatic micro-spraying equipment to fix the pured syphilis recombination antigen and anti-HBS single-clone antibody on a qualified nitrocellulose filter then to mix them labelled with colloidal gold in certain proportion to prepare a golden combined pad attached by proper sample process pads to form test paper slip, which quickly tests the syphilis antibody or hepatitis B surface antigen in a person blood, serum or plasma by double-antigen sandwich and double antibody sandwich methods.

Description

technical field [0001] The invention belongs to the category of pharmaceutical biological products and in vitro diagnostic reagents. It is a new combined rapid detection reagent that can detect whether a patient is infected with syphilis or hepatitis B virus with a drop of blood. Background technique [0002] Colloidal gold labeling and detection technology is relatively mature. The existing commercialized reagents detect syphilis or hepatitis B surface antigen separately, but there is no report of combined detection reagents. [0003] As we all know, the incidence of syphilis and hepatitis B in my country is on the rise, and the reform of blood donation policy in my country, the street blood collection vehicle of the blood center is very important for the physical examination of voluntary blood donors, which requires a simple, fast and specific , high accuracy, and a small amount of peripheral blood (50 μL) can detect several diseases with high incidence rates. The combin...

Claims

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Application Information

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IPC IPC(8): G01N33/549G01N33/558G01N33/569
Inventor 熊晓云耿永尧张文陈立炎
Owner 深圳益生堂生物企业有限公司
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