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Method for setting up dioecism type mussel inbred line

A method of establishment and a technology of allotypes, applied in the field of shellfish seed production, to achieve the effects of easy popularization and application, faster purification speed, and faster variety breeding speed

Inactive Publication Date: 2005-06-22
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no one has been found to use the hermaphroditic living bodies obtained from the above species to establish inbred lines. After years of research, the applicant of the present invention has finally successfully developed and completed the method for establishing inbred lines in dioecious shellfish.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Ezo scallop is a typical dioecious shellfish. Its gonads are horn-shaped after development. The female gonads are orange-red and the males are milky white. The sex of Ezo scallops can be easily distinguished from the appearance of the gonads.

[0030] In February of a certain year, 12,230 scallops with mature gonads were investigated in the sea area of ​​Changhai County, Dalian, and 81 scallops with hermaphroditic characteristics were found. The shells of these scallops are 10-13cm high and have a common feature: that is, there are areas of orange red and milky white in the gonads of each individual, and the sum of the surface areas of the milky white areas accounts for the total surface area of ​​the gonads. The ratio of the two colors varies widely, ranging from 0.1% to about 80%. The gonad tissues of the two colors are embedded with each other, but the boundaries between the two colors are clear and distinct. Individuals with different proportions of male gonad surfa...

Embodiment 2

[0061] After the Ezo scallops with hermaphroditic characteristics and large milky white areas are retrieved from the sea, carefully remove the attachments on the shell surface and wash them clean. They are temporarily raised in sand-filtered seawater for 12 days, during which time they are fed with single cells such as Phaeodactylum tricornutum. Algae are used as bait, and the water is changed twice a day, 1 / 2 each time, and the full amount of water is changed once every three days, and the temporary water temperature is 6°C.

[0062] h 2 、H 23 、H 24 、H 10 It is a hermaphroditic scallop with a large milky white area. The surface area of ​​the milky white area accounts for 15%, 20%, 30%, and 50% of the total surface area of ​​the gonads, and the H 2 and H 10 Same as Example 1, it is ready for use after ripening-accelerating cultivation, and the cultivation water temperature is 6°C. The operation process of establishing an inbred family is basically the same as in Example 1...

Embodiment 3

[0079] Ezo Scallop H 30 and H 31 is a scallop with a small male gonad area, H 30 There is only one milky white spot at the horn-like tip of the gonad, and the area of ​​the male gonad accounts for 0.1% of the total gonad surface area, H 31 Then there is a nearly round milky white plaque at the side edge of the gonads, accounting for 0.5% of the total surface area of ​​the gonads. The method of routine induced labor is basically the same as that of Example 1, after drying in the shade for 30 minutes, induce labor in 10 liters of preheated ultraviolet seawater, H 30 and H 31 Gamete discharge started after 30 and 55 minutes of immersion, respectively. After entering the water for 1.5 hours, take out the seed shells, carefully stir the water body to suspend the eggs and have ample opportunity to combine with the sperm. After 10 minutes, take a sample and check under the microscope to find H 30 Sperm, H 31 Sperm can be seen surrounding 85% of the eggs.

[0080] Carefully rem...

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Abstract

The invention provides a method for setting up dioecism type mussel inbred line which consists of selecting hermaphroditic special individuals from gonochorite seashells, establishing and cultivating selfing lines through the strategy of self crossing, the invention can be applied for accelerating the breed choosing and cultivating speed of gonochorite shells.

Description

technical field [0001] The invention relates to the seed production technology of shellfish, and in particular provides a method for establishing an inbred line of dioecious shellfish. Background technique [0002] Shellfish are mostly dioecious, a few species are hermaphroditic, and some species are sexually unstable. Dioecious shellfish are dioecious at the species level, that is, under normal circumstances, an individual has only one type of gonad, female or male, and after the gonad matures, an individual can only produce female (egg) or male (sperm) ) in a gamete; hermaphroditic shellfish are hermaphroditic at the species level, that is, each individual has two types of gonads, female and male, at the gonad development stage, and each individual can produce gonads when the gonads mature. Two gametes, female and male. [0003] In the clambranchs, only a few species such as the large scallop and the bay scallop in the genus Scallop are hermaphroditic species, and the va...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01K67/033
CPCA01K67/033A01K2227/70Y02A40/81
Inventor 刘晓张国范
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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