Serological assay for neospora caninum

A technology of Neospora and serum, applied in the field of serological detection, can solve the problem of not developing diagnostic tests and methods, etc.

Inactive Publication Date: 2005-07-13
PFIZER PRODS ETAT DE CONNECTICUT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no diagnostic test or method has been developed to date that can distinguish Neospora-naturally seropositive animals from Neospora-vaccinated and / or Neospora-seronegative (unvaccinated) animals

Method used

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  • Serological assay for neospora caninum
  • Serological assay for neospora caninum
  • Serological assay for neospora caninum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Antisera from Neospora seronegative animals and Neospora inoculated animals

[0034] Serum samples from any seronegative or PCR negative animal that subsequently received a modified live, inactivated or subunit based vaccine against Neospora tachyzoites may be used. In this example, 4-month-old calves were seronegative as determined by a diagnostic ELISA test by a licensed veterinary diagnostic laboratory (Washington State Diagnostic Lab, Pullman, WA). The calves were treated with a tachyzoite-based i) modified, live, attenuated Neospora vaccine (USPTO Patent Application No. 09 / 260,414 "Live Attenuated Neospora Vaccine" at days 0 and 21, here incorporated by reference), or ii) inactivated, whole cell homogenate Neospora vaccine (USPTO Patent Application No. 09 / 138,985 "Neospora Vaccine", incorporated herein by reference) vaccination. Both vaccines are based on the tachyzoite antigen as the protective component of the vaccine. The modified, live, attenuated vaccine a...

Embodiment 2

[0036] Antisera from seropositive animals naturally infected with Neospora

[0037] Serum samples from any naturally infected, seropositive or PCR positive animal can be used. In this example, the serological status of each animal in the purchased herd was determined using a diagnostic ELISA test by a licensed veterinary diagnostic laboratory (Washington State Diagnostic Lab, Pullman, WA). Twenty-one animals reported as seropositive by this test were identified. Serum samples from each seropositive animal were collected, pooled, and aliquoted and stored frozen at -20°C until serological assay testing (see Example 4).

Embodiment 3

[0039] Bradyzoite antigens used in neospora serological tests

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PUM

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Abstract

The present invention relates to the serological detection of animals inoculated with Neospora by reacting the test serum with a protein reactive with Neospora. The protein may be a full-length natural or recombinant Neospora or T. gondii bradyzoite fusion protein or a truncated fusion protein or a fragment thereof. The protein can be used in any of a number of assays including enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), Western blot and other suitable forms of immunoassay.

Description

field of invention [0001] The present invention relates to the serological detection of Neospora caninum infection by using the method of reacting test serum with protein capable of reacting with Neospora caninum. Background of the invention [0002] Neospora and Toxoplasma gondii are closely related pathogenic protozoan parasites in many species of animals. Neospora has been implicated as a cause of abortion, neurologically related limb defects and neonatal mortality in cattle and other animals. T. gondii is a common cause of abortion in sheep. Effective control of neosporosis and toxoplasmosis requires rapid diagnosis. It is therefore important to develop effective diagnostic tests against Neospora. [0003] There are several assays known in the art that can determine whether an animal has been exposed to Neospora. However, no diagnostic test or method has been developed to date that can distinguish Neospora naturally seropositive animals from Neospora vaccinated and / o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/45G01N33/53C12Q1/68G01N33/543G01N33/569
CPCG01N33/56905G01N2333/44G01N2333/45G01N2469/20G01N33/569
Inventor 戴维·A·布雷克黛安娜·M·里特
Owner PFIZER PRODS ETAT DE CONNECTICUT
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