Constructing and selecting method for lumbricine geminated maize stable inbred line

An inbred, stable technology, applied in the fields of botanical equipment and methods, applications, plant genetic improvement, etc., can solve problems such as instability

Inactive Publication Date: 2005-09-07
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, there are still a series of problems about how to effectively breed the opposite mutants obtained by the method of low-energy ion beam mutagenesis into other stable background lines, and then apply to the breeding of opposite hybrid combinations, including: The traits are controlled by multiple pairs of dominant and recessive interaction genes, and the opposite offspring are often unstable
[0003] The creation of new maize germplasm is an important way to breed breakthrough new varieties of maize. So far, the direct application of low-energy ion beams to maize mutagenesis has not been reported

Method used

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Experimental program
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Embodiment Construction

[0014] About the creation of opposite inbred lines in maize:

[0015] Use low-energy ion beams to inject corn inbred lines to create opposite inbred lines;

[0016] In the specific implementation, 30KeV, 6×10 15 N + / cm 2 N + The opposite mutant was injected into maize with the peeled off seed coat, and the opposite mutant obtained in the second generation was self-crossed and directed for 6 generations to obtain a stable opposite line H4 D , the leaves and ears of the opposite plants are opposite.

[0017] About the breeding of stable opposite inbred lines under different genetic backgrounds:

[0018] The inbred lines were selected by hybridization and backcrossing, and the presence of extra satellites on the third chromosome of the paired strains was detected by cytology as a sign of stable opposite traits, so as to breed different stable opposite inbred lines.

[0019] The specific implementation is based on H4 D Crossbreed with other alternate inbred lines, use othe...

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Abstract

Disclosed is a constructing and selecting method for lumbricine geminated maize stable inbred line characterized in that, injecting low energy ion beams into maize inbred lines, creating opposite inbred lines, selecting opposite inbred lines by means of hybridizing and backcrossing, using the third chromosome additional trabant existence as the symbol of the opposite property stability through cytology detection. The invention can be applied for obtaining stabilized maize opposite inbred lines.

Description

Technical field: [0001] The invention relates to a method for establishing and breeding a stable inbred line of opposite maize. Background technique: [0002] Low-energy ion beam is a new mutagenesis source, which has the characteristics of light damage, wide mutation spectrum and high mutation rate, and has been applied to the mutation breeding of various plants. However, there are still a series of problems about how to effectively breed the opposite mutants obtained by the method of low-energy ion beam mutagenesis into other stable background lines, and then apply to the breeding of opposite hybrid combinations, including: The traits are controlled by multiple pairs of dominant and recessive interacting genes, and the opposite offspring are often unstable. [0003] Creating new maize germplasm is an important way to breed breakthrough new varieties of maize. So far, the direct application of low-energy ion beams to maize mutagenesis has not been reported. Contents of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 程备久李展朱苏文蒋培东刘正
Owner ANHUI AGRICULTURAL UNIVERSITY
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