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Breeding method of clubroot-resistant orange-heading Chinese cabbage new germplasm

A technology for resisting clubroot and Chinese cabbage, applied in the fields of botany equipment and methods, biochemical equipment and methods, microbe measurement/inspection, etc., can solve problems such as orange leaf bulbs and clubroot resistance that have not been seen yet, Achieve the effects of not being easily affected by the environment and shortening the breeding period

Pending Publication Date: 2021-06-11
NORTHWEST A & F UNIV
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  • Description
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AI Technical Summary

Problems solved by technology

[0005] So far, there have been no reports on the application of molecular marker-assisted selection of orange leaf bulbs and clubroot resistance

Method used

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  • Breeding method of clubroot-resistant orange-heading Chinese cabbage new germplasm
  • Breeding method of clubroot-resistant orange-heading Chinese cabbage new germplasm
  • Breeding method of clubroot-resistant orange-heading Chinese cabbage new germplasm

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Effect test

Embodiment

[0068] A preferred embodiment of the present invention provides a method for breeding new germplasm of resistant clubroot orange Chinese cabbage, the specific steps are as follows:

[0069] 1. F 1 ;

[0070] 2. Plant F in the spring of the following year 1 (15ZF4) F 2 , planting F 2 The isolated population 15ZF4-2 has a total of 205 plants; the Chinese cabbage is planted on a flat land with uniform fertility and the soil is alkaline soil. Sow in plug trays and transplant to the open field after 30 days. The spacing between plants and rows is 45cm×50cm, and conventional field management is adopted;

[0071] At the seedling stage of Chinese cabbage, insert the card number of a single plant in the field, sample a single plant, and extract DNA; add 750uL of 1×CTAB extract to a 1.5ml centrifuge tube, the composition of which is: CTAB 2%, Tris-HC1 100mmol / L, EDTA 20mmol / L, NaCl1.4mol / L, and add 8μL of β-mercaptoethanol to shake well; take 0.2g of fresh tender leaves from each ...

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Abstract

The invention discloses a breeding method of a clubroot-resistant orange-heading Chinese cabbage new germplasm. The breeding method comprises the following steps that (1) an orange Chinese cabbage taken as a female parent and a clubroot-resistant Chinese cabbage taken as a male parent are hybridized to obtain F1, F1 single plants are selfed to obtain F2, an F2 group is planted, and DNA of the single plants is extracted; (2) the gardening shape of F2 is observed, ball cutting is conducted, and ball color phenotypic character observation and statistics are conducted; (3) PCR amplification is conducted by using a dominant orange gene marker Br530 and a clubroot-resistant marker SC2930-T / SC2930Q, and the genotype of the single plants is identified; (4) comprehensive evaluation is conducted according to a marker detection result and gardening shape observation, and a double-site homozygous clubroot-resistant orange-heading Chinese cabbage plant is selected to perform continuous single-plant selfing for two generations; and (5) selfing is conducted for three generations to obtain the clubroot-resistant orange-heading Chinese cabbage new germplasm. The single plants of Chinese cabbage segregation population are screened through molecular marker-assisted selection and field character selection, Chinese cabbages containing Chinese cabbage orange genes and clubroot-resistant genes at the same time are screened out, and high-quality germplasm resources are created for Chinese cabbage breeding.

Description

technical field [0001] The invention belongs to the field of vegetable variety breeding and germplasm resource innovation, and in particular relates to a new germplasm breeding method of clubroot-resistant orange Chinese cabbage. Background technique [0002] Chinese cabbage (Brassica campestris syn.rapa L.ssp.pekinensis) is one of the important vegetable crops of Brassica genus Brassica. With the improvement of people's living standards, the attention to the quality of Chinese cabbage is gradually increasing. Chinese cabbage requires High-quality Chinese cabbage must also be disease-resistant. However, high-quality Chinese cabbage is susceptible to disease. The use of pesticides will not only pollute the environment, but also make vegetables resistant to pesticides if used for a long time. Therefore, high-quality disease-resistant Chinese cabbage germplasm innovation become a research hotspot in recent years. [0003] In recent years, the orange Chinese cabbage cultivated ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04C12Q1/6895
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13A01H1/1255A01H6/204A01H5/12
Inventor 张鲁刚卢倩倩孟艳张妮南王亚秀和禹廷张学芬刘小愿
Owner NORTHWEST A & F UNIV
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