A kind of breeding method of male sterile line and maintainer line of sunflower with nucleoplasmic interaction

A male sterile line and maintainer technology, which is applied in the fields of botany equipment and methods, plant gene improvement, application, etc., can solve the problems of sunflower material defects such as outstanding defects, and achieve stable, fast, and stable sterile lines and maintainer lines. excellent effect

Active Publication Date: 2022-05-27
ECONOMIC CROPS RES INST XINJIANG ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0010] When the present invention is used for the breeding of new sunflower materials, it can solve the problem of prominent defects in a certain trait in sunflower materials in a targeted manner, and quickly and efficiently create new sunflowers through existing materials or collected resources with prominent advantages. Excellent breeding materials, followed by the rapid introduction of new varieties required by the market

Method used

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  • A kind of breeding method of male sterile line and maintainer line of sunflower with nucleoplasmic interaction
  • A kind of breeding method of male sterile line and maintainer line of sunflower with nucleoplasmic interaction
  • A kind of breeding method of male sterile line and maintainer line of sunflower with nucleoplasmic interaction

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Embodiment

[0052] figure 2 It is the principle of the breeding method for the nucleocytoplasmic interaction sunflower male sterile line and maintainer line provided by the embodiment of the present invention, which specifically includes:

[0053] Take the own maintainer line B{N(rr)} as the target genetic receptor, the heterozygous plant (A×R){S(Rr')} as the bridge, and replace the gene containing (r'r') in each generation The higher-grade plants, the sterile line A{S(r'r')} is the target genetic donor.

[0054] first generation F 1 , choose one maintainer line B{N(rr)} (r' accounts for 0%), target gene sterile line A{S(r'r')} (r' accounts for 100%) and sterile line heterozygote F 1 : (A×R){S(Rr')}(r' accounts for 50%) and each row is planted side by side; bagging when flowering, using (A×R){S(Rr')}50% of the pollen to artificially remove The male maintainer line B{N(rr)} is 0% pollinated, and the sterile line A{S(r'r')} is also 100% pollinated;

[0055] second generation F 2 , the...

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Abstract

The invention belongs to the technical field of plant breeding, and discloses a method for breeding male sterile lines and maintainer lines of sunflower with nucleoplasmic interaction, using the self-owned maintainer line B{N(rr)} as the target genetic receptor; heterozygous The plant (A×R){S(Rr')} is the bridge; the sterile line A{S(r'r') is the target genetic donor; the nuclear gene of the sterile line is continuously transferred to the maintenance type through the bridge In the plants, it finally contains more than 99% of the sterile line nuclear gene (r'r'), and finally becomes the matching maintainer line B{N(r'r')} of the sterile line. The invention enriches the types of the existing sunflower cytoplasmic male sterility maintaining lines and the method for transferring new sterility maintaining lines. The sunflower inbred line selected by the invention is stable, and the sterile line has complete sterility, and provides new germplasm resources for the utilization of sunflower heterosis. The new material created is stable and has excellent properties, and the purity of hybrid seeds assembled by using the new material can reach 100%, and the popularization and application effect is obvious.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for breeding male sterile lines and maintainer lines of nucleoplasmic interaction sunflower. Background technique [0002] Heterozygous crop plants have long been found to have natural advantages, and many crops are currently breeding hybrid varieties to improve yield and resistance. The discovery of the male sterile line has made hybrid breeding a step further, because it eliminates the need for manual emasculation, which not only saves manpower, reduces the cost of seeds, but also increases the possibility of obtaining seeds of higher purity. Breeding methods have been valued by many breeders. The use of crop male sterility to carry out cross-breeding has also become an effective way to utilize heterosis. The "three-line method" (restorer line, maintainer line and sterile line) of male sterility with nucleocytoplasmic interaction is one of the most...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 雷中华黄启秀向理军张黎顾元国侯献飞
Owner ECONOMIC CROPS RES INST XINJIANG ACAD OF AGRI SCI
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