A kind of breeding method of male sterile line and maintainer line of sunflower with nucleoplasmic interaction
A male sterile line and maintainer technology, which is applied in the fields of botany equipment and methods, plant gene improvement, application, etc., can solve the problems of sunflower material defects such as outstanding defects, and achieve stable, fast, and stable sterile lines and maintainer lines. excellent effect
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[0052] figure 2 It is the principle of the breeding method for the nucleocytoplasmic interaction sunflower male sterile line and maintainer line provided by the embodiment of the present invention, which specifically includes:
[0053] Take the own maintainer line B{N(rr)} as the target genetic receptor, the heterozygous plant (A×R){S(Rr')} as the bridge, and replace the gene containing (r'r') in each generation The higher-grade plants, the sterile line A{S(r'r')} is the target genetic donor.
[0054] first generation F 1 , choose one maintainer line B{N(rr)} (r' accounts for 0%), target gene sterile line A{S(r'r')} (r' accounts for 100%) and sterile line heterozygote F 1 : (A×R){S(Rr')}(r' accounts for 50%) and each row is planted side by side; bagging when flowering, using (A×R){S(Rr')}50% of the pollen to artificially remove The male maintainer line B{N(rr)} is 0% pollinated, and the sterile line A{S(r'r')} is also 100% pollinated;
[0055] second generation F 2 , the...
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