Breeding method of male sterile line and maintainer line of nucleo cytoplasmic interaction sunflower

Abstract

The invention belongs to the technical field of plant breeding, and discloses a breeding method of a male sterile line and a maintainer line of a nucleo cytoplasmic interaction sunflower. The method comprises the following steps: an own maintainer line B{N(rr)} is taken as a target genetic gene receptor; a hybrid plant (A*R){S(Rr')} as a bridge; a sterile line A{S(r'r') is taken as a target genetic gene donor; and nuclear genes of the sterile line are continuously transferred into the plants of the maintaining type through bridging, so that the plants finally contain 99% or more of the nuclear genes (r'r') of the sterile line, and finally, the matched maintainer line B {N(r'r'} of the sterile line is obtained. According to the invention, the types of the existing sunflower cytoplasmic male sterility maintainer line are enriched, and a new sterile maintainer line transformation method is provided. The sunflower inbred line bred by the invention is stable, the sterility of the sterile line is thorough, and a new germplasm resource is provided for the utilization of sunflower heterosis. The created new material is stable and excellent in character, the purity of hybrid seeds prepared from the new material can reach 100%, and the popularization and application effects are obvious.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, in particular to a method for breeding male sterile lines and maintainer lines of sunflower with nucleoplasmic interaction. Background technique [0002] Heterozygous crop plants have been found to have natural advantages very early on. At present, many crops are breeding hybrid varieties to improve yield and resistance. The discovery of the male sterile line has made hybrid breeding a step further, because it eliminates the need for artificial detasseling, which not only saves manpower, reduces the cost of seeds, but also increases the possibility of obtaining higher purity seeds. Breeding methods have been paid attention to by many breeders. The use of male sterility in crops for cross breeding has also become an effective way to utilize heterosis. The "three-line method" (restorer line, maintainer line and sterile line) of nuclear-cytoplasmic interaction male sterility is one of the m...

AI Technical Summary

Problems solved by technology

[0010] When the present invention is used for the breeding of new sunflower materials, it can solve the problem of prominent defects in a certain trait in sunflower materials in a targeted manner, and quickly and efficiently create new sunflowers through existing materials or collected resources with prominent advantages. Excellent breeding materials, followed by the rapid introduction of new varieties required by the market

Images