Extract with anti-tumor and anti-poisonous activity
An extract and active technology, applied in the medical field, can solve problems such as limiting long-term treatment
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Embodiment 1
[0220] Embodiment 1 extracts the method for the extract of the present invention from plant white-flowered horned melon
[0221] The extract of the present invention (Extract A) was extracted with methanol from the root of the crocus plant (Romoaceae). The extract is also referred to as Extract A (number). Put 10 g of plants into a Erlenmeyer flask containing 150 ml of methanol, stir with a magnetic force for 12 hours, and then filter through fritted glass. The solid residue was extracted with methanol for an additional 2 hours. The two filtrates were combined and evaporated under vacuum using a rotary evaporator. The residue containing the methanolic extract was named Extract A.
[0222] Extract A was analyzed to contain several types of compounds. There is a special group of compounds in the extract A consisting of cardiotonic steroids, such as ashalogenoside, calactin, vorusharin, acrotoxin, acrogenin, ousalogenin, calotoxin, usharin and usharidin. In addition, other ...
Embodiment 2
[0234] Example 2 Effect of the extract of the present invention on the overall growth of cell line cells
[0235] This example illustrates the antitumor activity of the extract A of the present invention on different types of tumors. In order to characterize the in vitro activity of Extract A, the MTT assay of Extract A was performed. The MTT assay is an indirect assay well known in the art that can rapidly, ie within 5 days, determine the effect of a given product on the overall growth of a cell line. The test can detect the number of metabolically active living cells, because living cells can reduce the yellow MTT product (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetra azole olfactory compound) into the blue product formazan, which can only be produced by living cells. The amount of formazan formed at the end of the test was measured by a spectrophotometer, and its value was proportional to the number of viable cells.
[0236] The 48 human tumor cell lines described in T...
Embodiment 3
[0254] Embodiment 3 The influence of the extract of the present invention on cell dynamics
[0255] This example illustrates the cell proliferation inhibitory effect of extract A of the present invention. From the results of the MTT colorimetric analysis described in Example 2, it can be clearly seen that the extract A can significantly inhibit the overall growth level of most of the 48 human cancer cell lines analyzed in the MTT test. In the following examples, it is necessary to analyze whether the inhibitory effect of the extract on cell growth is caused by changes in cell cycle dynamics (Example 3), or due to the induction of apoptosis (Example 4), or both factors exist.
[0256] The cell cycle is generally divided into the following phases: G0 / G1, S and G2 / M phases. Alterations in proteins that regulate cell proliferation and / or cell death are the targets of the active compounds in Extract A. Changes in cell cycle kinetics can be measured by flow cytometry with differe...
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