Method for building animal model of forming thrombus of deep vein in traumatic limbs and trunk of rat

A technology for deep vein thrombosis and its establishment method, which can be applied to preparations and pharmaceutical formulations for in vivo experiments, can solve problems such as damage to blood vessels, and achieve low animal mortality, high incidence of deep vein thrombosis, and simple and easy production process. Effect

Inactive Publication Date: 2006-02-01
昆明医学院第一附属医院
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AI Technical Summary

Problems solved by technology

[0006] Although the above modeling methods have their own characteristics, they are mainly used to study changes in blood coagulation and fibrinolytic systems, as well as related drug screening and curative effect evaluation, which are not consistent with the actual natural course of clinical DVT in orthopedics: (1) Absolute clinical DVT in orthopedics Most of them are subacute or chronic, and the thrombus formed by the above methods are all acute; (2), orthopedic clinical DVT hardly directly damages deep veins, and the above methods all directly damage blood vessels
(3) Most of the orthopedic clinical DVT occurs in patients with immobilized limbs after trauma or surgery and long-term bed rest. DVT occurs despite anticoagulants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] 1. Modeling process and observation indicators

[0033] 1. Animals and groups

[0034] 120 Wistar rats, male or female. The weight ranged from 230g to 300g. After two weeks of adaptive feeding, they were randomly divided into three groups: A, B, and C: group A was the fracture group, group B was the trauma group, and group C was the control group. 40 rats in each group were randomly divided into 3-day observation group, 5-day observation group, 7-day observation group and 9-day observation group according to different observation time points after modeling.

[0035] 2. Self-made hitting device

[0036] Based on the orthopedic intramedullary nail driver, it is marked with a scale and can be struck with hammers of different qualities. Trauma energy was calculated by Ep=mgh. The diameter of the hitting contact disc is 1 cm.

[0037] 3. Modeling method

[0038] Rats were intraperitoneally injected with 1% pentobarbital sodium 1ml / kg. After the anesthesia took effect, ...

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Abstract

A method for creating the animal model of traumatic deep venous thrombosis in limbs features that the intramedullary nail driver is reformed to become a quantitative beater and said beater is used to beat the near ends of lower limbs of rat to obtain said animal model.

Description

technical field [0001] The invention relates to the technical field of medical models, and is a method for establishing an animal model of traumatic limb deep vein thrombosis with orthopedic clinical characteristics, which can be used to study the occurrence, development and outcome of traumatic limb deep vein thrombosis and pulmonary thromboembolism, and explore more effectively The method of prevention, diagnosis and treatment provides an animal model platform. Background technique [0002] Thrombosis is a kind of disease that seriously endangers human health and life. It is found in patients in many clinical departments. Its morbidity, disability and mortality are very high, and it is extremely harmful. In western countries, the incidence rate of venous thromboembolism (VTE) is as high as 0.1%, and half of them are deep venous thrombosis (DVT). In the United States, there are about 650,000 hospitalizations and 50,000-200,000 deaths each year due to DVT and pulmonary thro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
Inventor 赵学凌李文黄河王兵赵宏斌何飞王建伟吴雪梅张春强贾代良赵智唐锡章周兆文李世和
Owner 昆明医学院第一附属医院
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