Quantitative detection reagent and kit for malt agglutinin receptor on sperm membrane surface

A quantitative detection and lectin technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as inability to clinical diagnosis, and achieve the effect of bright colors, easy interpretation, and intuitive results.

Inactive Publication Date: 2012-02-01
深圳华康生物医学工程有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, in the prior art, the main method for detecting malt agglutinin receptors on the surface of sperm membrane is nephelometry, which is limited to laboratory research due to the limitation of experimental conditions and technical reasons, and cannot be really popularized and applied in clinical diagnosis.

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  • Quantitative detection reagent and kit for malt agglutinin receptor on sperm membrane surface
  • Quantitative detection reagent and kit for malt agglutinin receptor on sperm membrane surface

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Embodiment Construction

[0038] The present invention will be described in further detail below through specific examples.

[0039] A kit for quantitative detection of malt agglutinin receptor on sperm membrane surface, comprising the following components:

[0040] (1) buffer solution, (2) fixative solution (3) washing solution (4) enzyme conjugate (5) chromogenic reagent A (6) chromogenic reagent B (7) special glass slide (4 wells / block)

[0041] The preparation process of each detection reagent is as follows:

[0042] (1), the preparation technology method of buffer solution:

[0043] 1), accurately weigh calcium chloride dihydrate (CaCl 2 2H 2 O) 0.132g, potassium chloride (KCl) 0.2g, potassium dihydrogen phosphate (KH 2 PO 4 )0.2g, disodium hydrogen phosphate (Na 2 HPO 4 ) 1.15g, sodium chloride (NaCl) 8.0g, thimerosal 0.2g, magnesium chloride hexahydrate (MgCl 2 ·6H 2 (0) 0.1g, placed in a beaker, added 800 milliliters of distilled water and stirred to dissolve;

[0044] 2) Adjust the p...

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Abstract

The invention discloses a reagent and kit for quantitative detection of malt agglutinin receptors on the surface of sperm membranes, and proposes a corresponding detection method to quantitatively detect malt agglutinin receptors on the surface of human sperm membranes and provide information on the location and quantity of WGA receptors on sperm membranes , directly applied to the clinical diagnosis of blindness, the detection method is easy to operate, batch detection can be carried out, and the detection time is greatly shortened.

Description

【Technical field】: [0001] The invention relates to an immunoenzyme cytochemical staining detection reagent. 【Background technique】: [0002] The malt agglutinin receptor on the sperm membrane surface plays an important role in spermatogenesis, maturation, capacitation and fertilization. Once the specific determinant on the sperm membrane recognizes the sperm receptor on the surface of the zona pellucida of the egg cell, it will trigger a series of chain processes such as acrosome reaction, and finally complete the fertilization. Studies have shown that sperm membrane malt agglutinin (WGA) receptors are closely related to sperm fertilization function, the absence of WGA receptors in ejaculated sperm is one of the causes of infertility, and changes in the location and number of WGA receptors can cause infertility or habitual abortion. Therefore, it can be used as a means of clinical diagnosis of unexplained infertility. However, in the prior art, the main method for detectin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/08G01N33/535
Inventor 傅剑华刘瑜胡家纯何林何小红
Owner 深圳华康生物医学工程有限公司
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