Potato rot nematode rDNA-ITS sequence, specific detection primer and one-step dual PCR detection method

A method of detection, technology of potatoes, applied in the field of biology
CN1763193AInactive Publication Date: 2006-04-26INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Publication Date
2006-04-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention is potato rot stem nematode rDNA-ITS sequence, specific detection primer and one-step double PCR detection method. The potato rot stem nematode rDNA-ITS has the nucleotide sequence as shown in SEQ No. 1 or SEQ No. 2. The specific detection primer SSsj designed based on the rDNA-ITS sequence and general purpose primer AB28 are combined and added with internal standard D3A and D3B for one-step double PCR detection. The PCR amplification result of potato stem nematode can obtain specific 623 bp segment and 345 bp segment, but that of other stem nematode can obtain specific 345 bp segment only. The present invention provides fast molecular detection method for stem nematode detection and identification and thus provides theoretic reference for comprehensive control of stem nematode disease.
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Description

Technical field:

[0001] The invention belongs to the field of biological technology, and relates to the rDNA-ITS sequence of D. destructor potato, a specific primer sequence designed for the sequence and a rapid PCR detection method for D. destructor potato by using the primer. technical background

[0002] D.potato rot nematode (D.destructor) which damages sweet potato and D. dipsaci which damages garlic, corms and bulbs are important diseases of crops in my country. Sweet potato stem nematode disease has been reported in 21 counties and cities including Lulong County, Changli, Luan County, Qian'an, Langfang, Bazhou, Quyang County and Dingzhou in Hebei, Miyun, Daxing, Fangshan in Beijing, Xuchang in Henan, Zhengzhou, Pingdingshan, Jia County, Jilin City, Jilin City, Liaoning, Shandong Yishui County, Jiangsu Xuzhou and other provinces and cities are common, and the disease is very serious, mainly infecting the underground roots, tubers and tubers of sweet potatoes, and the d...

Claims

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