Wall breaking method of microalgae cell

A technology of microalgae cells and microalgae is applied in the field of microalgae cell wall breaking technology rich in biologically active substances, which can solve the problems of loss of biologically active components, long use time, and high temperature, so as to increase work efficiency, avoid damaging effects, The effect of not polluting the environment

Inactive Publication Date: 2006-06-14
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are obvious defects in the above methods, such as long time, high temperature, poor pulverization effect, or no effective

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Grinding of Haematococcus wet material by high pressure homogenization. Add a food protectant (the food protectant in this example is 2,6-di-tert-butylcresol (BHT)) to the wet material of Haematococcus, and mix the dry weight of the material and the protectant in a weight ratio of 99:1. Then the wet material is pretreated at low temperature, the temperature is lowered to about 4°C, and the high pressure between 400 bar and 1500 bar is used to push the material into the working chamber. Then the wet material loses pressure instantly through the flow-limiting slit of the high-pressure homogenizer to the normal pressure state, and the depressurized microalgae material is ejected rapidly at 3 times the speed of sound. Combined with the shearing effect, the cell wall of the wet material microalgae is broken in an instant, and the cell wall breaking effect is very obvious. The particle size of the material is almost all refined to a micron material with a particle size below...

Embodiment 2

[0053] High-velocity gas crushing of Haematococcus dry material. Add a food protectant (the food protectant in this example is 2,6-di-tert-butylcresol (BHT)) to the dry material of Haematococcus, and mix the dry weight of the material and the protectant in a weight ratio of 99:1. Then freeze the dry material of Haematococcus, and cool the material temperature to below -20°C. Then, driven by the compressed air, it enters the working area of ​​the cylindrical cavity at high speed along the tangential direction, and continues to accelerate the movement, finally reaching 3 times the supersonic speed, resulting in a violent collision between the high-speed Haematococcus material particles and the low-speed particles, which can be achieved within a few seconds. Instantly produces cell wall breaking effect. Among them, smaller particles of Haematococcus material are discharged and collected from the center of the working cavity along with the gas. The temperature of the Haematococc...

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PUM

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Abstract

The invention belongs to micro-algae biotechnology field. It relates to micro-algae cell breaking wall method. It includes three parts technique: wet method micro-algae mud cell breaking wall technique, dry method micro-algae powder cell breaking wall technique, and the corresponding method of protecting micro-algae biological activity component, reducing its oxidative decomposition. The micro-algae material is added an amount of preservative to form isotropic protective layer, utilized high-speed fluid grain strength collision to finish breaking cell wall. The invention has the advantages of quick speed, high efficiency, non foreign matter, and effectively protecting biologically active substance.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, in particular to the microalgae cell wall breaking technology rich in bioactive substances. Background technique [0002] As an important part of primary productivity in the biosphere, microalgae are the only bait for the entire growth process (or specific developmental stages) of aquatic animals in the aquatic ecosystem or the bait for bait organisms. At the same time, microalgae are close to 50% of plant species, and have diversity, complexity and particularity in terms of germplasm, ecological distribution, genetic information, biochemical composition, and metabolic pathways, which further determine their potential food, nutrition, health care and Pharmaceutical equivalents. It is entirely possible for human beings to develop high-value-added bioactive ingredients that are in great demand and structurally specific (such as β-carotene, astaxanthin, lutein and other carotenoids and their...

Claims

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Application Information

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IPC IPC(8): C12N1/12
Inventor 刘建国刘伟林伟邢军武
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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