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Method for screening of immunological tolerance associated disease gene RGS1 based on immunological tolerance dendritic cell

A technology of dendritic cells and immune tolerance, applied in blood/immune system cells, animal cells, vertebrate cells, etc.

Inactive Publication Date: 2006-10-04
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no systematic approach to identify genes controlling immune tolerance in dendritic cells

Method used

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  • Method for screening of immunological tolerance associated disease gene RGS1 based on immunological tolerance dendritic cell
  • Method for screening of immunological tolerance associated disease gene RGS1 based on immunological tolerance dendritic cell
  • Method for screening of immunological tolerance associated disease gene RGS1 based on immunological tolerance dendritic cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Preparation of immune tolerant dendritic cells.

[0073] Specific preparation method:

[0074] 1.1. Experimental materials:

[0075] 1) The medium is PRMI1640 medium (Hyclone Company) containing 10% calf serum (Minhai Biological Company), and 1% penicillin and 1% streptomycin (Sigma Company) are added at the same time. The experimental temperature condition is 37°C. The culture medium in the following description is this culture medium.

[0076] 2) Ovarian tumor cells are tumor cells isolated from mouse ovarian tumor tissues; ovarian tumor cells were provided by Dr. Katherine Roby (Carcinogenesis, 21:585-591, 2000). To determine whether the induction of tumor-associated immune tolerance dendritic cells is through direct cell-to-cell contact or factors secreted by the tumor cells themselves, we treated ovarian cancer cells as follows:

[0077] A. Use 14000 Rad Co. 60 Irradiate ovarian cancer cells. Such cells were used to evaluate the effects of direct cell-to-cell...

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Abstract

The provided screen method for RGS1 gene on immune tolerance comprises: preparing human immune tolerance dendritic cell by external culture to increase the expression of RGS1 gene in the dendritic cell; when transfecting siRNA of RGS1 gene or aimed genes with monocyte series, affecting activity of transcription factor and expression; and playing special immunoregulation function to guide immune tolerance. This invention has great application potential.

Description

technical field [0001] The invention relates to a method for inducing immune tolerance dendritic cells in vitro to screen the gene RGS1 of immune tolerance-related diseases, specifically the gene regulator of G protein 1 (hereinafter referred to as the RGS1 gene), the known new function of the gene RGS1 Discovered screening and identification methods, RGS1 is involved in immune tolerance and affects the expression of immune response genes. RGS1 gene has potential clinical application value in the treatment of immune-related diseases such as anti-transplant rejection, tumor, inflammation and autoimmune diseases. Background technique [0002] In 1975, the famous immunologist Burnet proposed the theory of clonal selection, and explained the phenomenon of immune tolerance with the theory of clonal deletion. The T and B reactive cell lines in the immature stage are cleared due to exposure to antigens, resulting in immune tolerance. However, current understanding of immune toler...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N5/08C12N5/0784
Inventor 杨荣存刘昱R.卢登W.如适张园张灼寒
Owner NANKAI UNIV
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