Multiple PCR reaction kit and detecting process thereof

Abstract

The invention discloses a PCR diagnostic reagent box for detecting the milk cattle foot rot. It has the advantage of quick, high sensibility, strong speciality and low cost.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a multiple PCR reaction kit for detecting cow foot rot and a detection method thereof. Background technique [0002] Foot rot is one of the main diseases that endanger the dairy industry. It is mainly an infectious disease caused by a variety of pathogenic bacteria concurrently infected with each other. Its incidence is relatively high, accounting for about 30% of foot and foot diseases. Foot rot occurs in dairy farms all over the country. If the disease is severe, the cows will lie down on the ground, the milk production will decrease, and the elimination rate will increase, which will bring great economic losses to the dairy industry. [0003] In China, the examination of foot rot is initially diagnosed through clinical symptoms and pathological changes, but it is difficult to distinguish hoofitis, interdigital dermatitis, and sole ulcer. The detection of foot rot in foreign count...

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Problems solved by technology

[0003] In China, the inspection of foot rot is initially diagnosed through clinical symptoms and pathological changes, but it is difficult to distinguish hoofitis, interdigital dermatitis, and sole ulcer

In foreign countries, ELISA and PCR detection methods have been used in the detection of foot rot. ELISA test has high specificity, but its sensitivity is very low, so it is not easy to popularize

The PCR method has the advantages of rapidity, accuracy, specificity, etc., but it is only designed and applied for the single pathogenic bacteria of B.

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