Pretreatment method for lily tissue culture bulbs before transplanting them

A lily group and pretreatment technology, applied in the field of plant tissue culture, can solve the problems of slow germination, weak growth, irregular seedling emergence, etc., and achieve the effects of vigorous seedling growth, strong stress resistance, and improved stress resistance.

Inactive Publication Date: 2007-01-31
潘利军
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  • Summary
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current lily tissue culture seedlings are directly transferred after simply reducing the humidity and hardening before transp

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example

[0011] Example 1 Method for low temperature treatment of lily tissue culture seedlings:

[0012] 1) Place lily bulblets with a diameter of 0.8~1.5cm (average 1.3cm) induced in the process of tissue culture under low temperature conditions of 0, 2, 4, 6, 8, and 10 ℃ respectively before being transplanted. After 30 days of treatment ,Transfer into the same matrix and observe its growth, as shown in Table 1 below;

[0013] Temperature(℃)

[0014] It can be seen from the situation of bulbs after transplantation in Table 1 that: low temperature treatment is very beneficial to the growth of tissue cultured seedlings after transplantation. As shown in Table 1, when the temperature is controlled at 2 to 4°C, after 30 days of treatment, lily After the bulbs are transferred, the time required for emergence is the shortest, and the neatness and growth of the seedlings are in the optimal state; when the temperature is lower than 2℃, as the temperature decreases, the emergence time w...

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Abstract

A method for pre-treating the tissue-cultured bulb of lily before transplanting features that the bulb of lily is treated at 2-6 deg.C for 30-60 days, resulting in high survival rate and strong seedling.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a pretreatment method before transferring lily tissue culture balls. Background technique [0002] Plant tissue culture refers to the method of inoculating a part of a plant, such as a small stem, a small leaf, or even a cell, on a medium under in vitro conditions to make them regenerate into a complete plant. The steps of tissue culture include disinfection of medium and inoculation material, inoculation, plant induction, rooting and transplanting. [0003] Present lily tissue-cultured seedlings are directly transferred after simply reducing the humidity to harden the seedlings before transplanting. After the tissue-cultured seedlings are transferred, germination is slow, seedlings are not neat, and their growth is weak. Contents of the invention [0004] The object of the present invention is to provide a pretreatment method for lily tissue culture balls with fast germination, neat se...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 潘利军
Owner 潘利军
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