Cow ovum cell in vitro ripening culturing liquid containing tea polyphenol and its culturing method

Abstract

The present invention provides an in vitro mature culture fluid containing tea-polyphenol for bovine ovocyte and its culture method. The described in vitro mature culture flud uses conventional culture fluid as matrix, in the described matrix 20-150 microgram / mL of tea-polyphenol, 100 IU / mL of penicillin, 100 microgram / mL of streptomycin and serum whose volume percentape content is 8%-20% are also contained. Said invention can make tea-polyphenol be used in the field of bovine ovocyte in vitro mature culture so as to raise early embryonic in vitro development rate after the bovine ovocyte in vitro fertilization.

Description

(1) Technical field

[0001] The invention relates to a bovine oocyte in vitro maturation culture solution containing tea polyphenols and a culture method thereof, belonging to the technical field of livestock embryo engineering. (2) Background technology

[0002] With the development of modern reproductive biotechnology and the gradual improvement of embryo in vitro production technology, the use of in vitro fertilization technology to produce in vitro embryos presents a potential broad application prospect. In vitro production of livestock embryos, especially somatic cell nuclear transfer, is an effective way to rapidly expand the number of excellent populations. A sufficient number of mature oocytes is a prerequisite for in vitro production of embryos. Extracting oocytes with a syringe from ovaries obtained from slaughterhouses and culturing them in vitro is the most effective and economical way to obtain mature oocytes. Since the concentration of oxygen in the reproducti...