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Plactycodon grandiflorous tetraploid breeding method

A cultivation method and tetraploid technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., to achieve the effects of robust plants, fast growth, and dark leaves

Inactive Publication Date: 2007-04-25
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestically, there have been studies on polyploid induction with callus of platycodon grandiflorum in order to cultivate varieties with high medicinal value, but there is no research on the use of colchicine for its test-tube plantlets on the basis of the introduction, domestication and selection of wild platycodon grandiflorum. Polyploid Induction in Shoot Tips to Breed Ornamental Polyploid Platycodon grandiflora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Cut the sturdy young buds of the domesticated and cultivated wild Chinese Platycodon grandiflorum, wash them with tap water, and put them in 0.1% mercury (Hgcl 2 ) Soak in the solution for 20 minutes, then put it in a 2% sodium hypochlorite solution for 12 minutes, rinse with sterile water 3 times, and inoculate the sterilized cuts into the unit under aseptic conditions In the following induction medium of mg / L:

[0023] MS basic medium

[0024] 6-Benzylaminopurine (6-BA) 0.1mg / L

[0025] Kinetin (KT) 0.05mg / L

[0026] Naphthalene acetic acid (NAA) 0.1mg / L

[0027] Sucrose 30000mg / L

[0028] Agar 4500mg / L

[0029] pH 5.5

[0030] Under the environmental conditions of 12h / d light, 1200Lx light intensity, and 25℃ temperature, cultivate for 20d to make adventitious buds directly form from the incision;

[0031] 2. Cut the formed adventitious bud clusters and put them into the following medium:

[0032] MS basic medium

[0033] 6-Benzylaminopurine (6-BA) 0.1mg / L

[0034] Kin...

Embodiment 2

[0050] 1. Cut the sturdy young buds from a single Chinese Platycodon grandiflorum, wash them with tap water, and put them in 0.2% mercury (Hgcl 2 ) Soak in the solution for 10 minutes, then put it in a 1% sodium hypochlorite solution for 15 minutes, rinse twice with sterile water, under aseptic conditions, inoculate the sterilized cut into the unit In the following induction medium of mg / L:

[0051] MS basic medium

[0052] 6-Benzylaminopurine (6-BA) 0.3mg / L

[0053] Kinetin (KT) 0.1mg / L

[0054] Naphthalene acetic acid (NAA) 0.3mg / L

[0055] Sucrose 30000mg / L

[0056] Agar 4500mg / L

[0057] pH 5.8

[0058] Under the environmental conditions of 10h / d light, 800Lx light intensity, and 22°C temperature, cultivate for 30d to make adventitious buds grow directly from the incision;

[0059] 2. Cut the formed adventitious bud clusters and put them into the following medium:

[0060] MS basic medium

[0061] 6-Benzylaminopurine (6-BA) 0.3mg / L

[0062] Kinetin (KT) 0.1mg / L

[0063] Naphtha...

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PUM

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Abstract

The invention relates to a method for cultivating Campanulales tetraploid, which uses the YunNan wide flower as cultivate material, via the polyploidy cultivate technique of vegetable, fruit, and other garden plants, to induce the sharp of campanulales, with high induce rate, and make the adventitious bud with significant change, and obtain short adventitious bud. The invention can stabilize and expand the tetraploid campanulales plants, to accelerate cultivation, while the product has large leaf and quick growth.

Description

Technical field [0001] The invention relates to a plant breeding method, in particular to a method for doubling the chromosome induction and rapid propagation and cultivation of plant cells under in vitro conditions, and belongs to the field of biotechnology breeding. Background technique [0002] Chinese Platycodon grandiflorus (Platycodon grandiflorus A.DC) belongs to Campanulaceae (Campanulaceae) Platycodon (Platycodon A.DC) perennial herb, native to Northeast my country, North China, East China, Central China provinces as well as Guangdong, North Guangxi, Guizhou, Southeast Yunnan ( Mengzi, Yanshan, Wenshan), Sichuan (Pingwu, east of Liangshan), Shaanxi. North Korea, Japan, the Far East of the Soviet Union and the southern part of Eastern Siberia also exist (Hong Deyuan et al. 1983). Platycodon grandiflorum has medicinal value. Its root is used as medicine. It has the functions of clearing the lungs, improving eyesight, pharyngeal, expectorating, relieving cough, relieving pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00C12N5/04
Inventor 屈云慧熊丽王小华瞿素萍李进昆
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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