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Plactycodon grandiflorous tetraploid breeding method

A cultivation method and tetraploid technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., to achieve the effects of obvious variation, fast growth, and speeding up the breeding process

Inactive Publication Date: 2009-07-08
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestically, there have been studies on polyploid induction with callus of platycodon grandiflorum in order to cultivate varieties with high medicinal value, but there is no research on the use of colchicine for its test-tube plantlets on the basis of the introduction, domestication and selection of wild platycodon grandiflorum. Polyploid Induction in Shoot Tips to Breed Ornamental Polyploid Platycodon grandiflora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Cut the robust young shoots of the domesticated and cultivated wild Platycodon grandiflorum single plant, clean them with tap water, and put in 0.1% mercury chloride (Hgcl 2 ) solution for 20 minutes, then put into 2% sodium hypochlorite solution and soak for 12 minutes, rinse 3 times with sterile water, and inoculate the sterilized cut into the unit under aseptic conditions. In the following induction media in mg / L:

[0023] MS Basic Medium

[0024] 6-Benzylaminopurine (6-BA) 0.1mg / L

[0025] Kinetin (KT) 0.05mg / L

[0026] Naphthaleneacetic acid (NAA) 0.1mg / L

[0027] Sucrose 30000mg / L

[0028] Agar 4500mg / L

[0029] pH 5.5

[0030] Under the environmental conditions of light 12h / d, light intensity 1200Lx, and temperature 25°C, cultivate for 20 days, so that adventitious buds can be directly formed from the incision;

[0031] 2. Cut the resulting adventitious bud clusters into the following medium:

[0032] MS Basic Medium

[0033] 6-Benzylaminopurine (6-BA...

Embodiment 2

[0050]1. Cut off the strong young shoots of Platycodon grandiflorum single plant, clean them with tap water, and put in 0.2% mercury chloride (Hgcl 2 ) solution for 10 minutes, then put into a 1% sodium hypochlorite solution and soak for 15 minutes, rinse twice with sterile water, and inoculate the sterilized cut into the unit under aseptic conditions. In the following induction media in mg / L:

[0051] MS Basic Medium

[0052] 6-Benzylaminopurine (6-BA) 0.3mg / L

[0053] Kinetin (KT) 0.1mg / L

[0054] Naphthaleneacetic acid (NAA) 0.3mg / L

[0055] Sucrose 30000mg / L

[0056] Agar 4500mg / L

[0057] pH 5.8

[0058] Under the environmental conditions of light 10h / d, light intensity 800Lx, and temperature 22°C, cultivate for 30 days, so that adventitious buds can be directly formed from the incision;

[0059] 2. Cut the resulting adventitious bud clusters into the following medium:

[0060] MS Basic Medium

[0061] 6-Benzylaminopurine (6-BA) 0.3mg / L

[0062] Kinetin (KT) 0.1...

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Abstract

The invention provides a method for cultivating tetraploids of Platycodon chinensis, which uses the characteristic wild flower of Platycodon chinensis in Yunnan as the breeding material, with the help of the polyploid breeding technology of vegetables, fruits and other horticultural plants, adopts autumn Narcisine treatment induces not only a high induction rate, but also makes the adventitious buds mutate very obviously, and the mutated buds are thick and short, thereby obtaining better results. At the same time, the present invention adopts the technology of rapid propagation of adventitious buds to stabilize and multiply in a short time. Tetraploid Platycodon grandiflora plants were obtained, the breeding process was accelerated, and polyploid Chinese Platycodon grandiflorum germplasm with high ornamental value or good utilization value could be bred. The leaves of platycodon polyploid tissue culture seedlings obtained by the method of the invention are obviously larger and thicker than diploid leaves, the leaves are darker in color, grow faster, and the plants out of the bottle are robust, initially reflecting the advantages of polyploid in form.

Description

technical field [0001] The invention relates to a plant cultivation method, in particular to a method for inducing plant cell chromosome doubling and rapid propagation and cultivation under in vitro conditions, belonging to the field of biotechnology breeding. Background technique [0002] Chinese bellflower (Platycodon grandiflorus A.DC) belongs to Campanulaceae (Campanulaceae) Campanulaceae (Platycodon A.DC) perennial herbaceous plant, native to my country's Northeast, North China, East China, Central China provinces and Guangdong, northern Guangxi, Guizhou, southeastern Yunnan ( Mengzi, Yanshan, Wenshan), Sichuan (Pingwu, east of Liangshan), Shaanxi. North Korea, Japan, the Far East of the Soviet Union and the southern part of East Siberia (Hong Deyuan et al. 1983). Platycodon grandiflorum has medicinal value. Its root is used as medicine. It has the functions of clearing lung and eyesight, pharynx, eliminating phlegm, relieving cough, relieving pain and dispelling stagna...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01H4/00C12N5/04
Inventor 屈云慧熊丽王小华瞿素萍李进昆
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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