Method for united sequential culture of human vaginal epithelial cell by using two kinds of culture medium

Abstract

The invention discloses a culturing method of combined sequential cell in the culturing technique scale of human vaginal epithelial cell, which is characterized by the following: adopting non-serum culture medium and serum culture medium to do sequential culture; breeding human vaginal epithelial cell rapidly; improving successful generation rate at 80%.

Description

technical field [0001] The invention belongs to the technical scope of cell culture, in particular to a method for sequentially culturing human vaginal epithelial cells in combination with two culture media. Background technique [0002] Since no domestic scholars have done the culture of human vaginal epithelial cells, we applied for a fund from the Beijing Municipal Science and Technology Commission as a source of funding for the establishment of human vaginal epithelial cell models and the co-culture experiment of epithelial cells and Candida albicans. The experiment adopted the tissue block method for primary cell culture, and keratinocyte serum-free medium (Keratinocyte-SFM, product of American GIBCO Company, No. 17005-042) was used as the main cell culture medium. However, it was found in the experiment that the initial growth rate of the primary cells cultured by K-SFM was slow, and the number of primary vaginal epithelial cells cultured by pure K-SFM was small, and p...

AI Technical Summary

Problems solved by technology

In foreign countries, there are also examples of the combined use of two media for culturing primary epithelial cells, but they are all cultured in serum-based media (2-7 days) and then cultured in serum-free media, which is similar to the direct use of serum-free media. Vaginal Epithelial cells are also difficult to passage

However, our method is the sequential culture of human vaginal epithelial primary cells in serum-free medium + serum-containing medium. At present, there is no report on obtaining a large number of human vaginal epithelial cells by this method.