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Composition for preventing or treating glioblastoma comprising Platycodon grandiflorum A. De Candolle, Scutellaria baicalensis, Phellodendron amurense Ruprecht or Rubus coreanus

a technology of glioblastoma and composition, which is applied in the direction of medical preparations, plant ingredients, plant/algae/fungi/lichens ingredients, etc., to achieve the effects of inhibiting the growth of gbm, preventing, improving, or treating gbm

Active Publication Date: 2022-06-07
UNIV IND COOP GRP OF KYUNG HEE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0015]The extract of Platycodon grandiflorum of the present invention, a fraction thereof, or the platycodin D (hereinafter, PD) contained therein can effectively treat glioblastoma multiforme (GBM) by inducing the expression of low-density lipoprotein receptors (LDLRs) through an anti-autophagic action; and the extract of Scutellaria baicalensis, Phellodendron amurense Ruprecht, or Rubus coreanus of the present invention or a fraction thereof can effectively inhibit the growth of GBM by inhibiting the expression of receptor tyrosine kinase (Ax1). Therefore, the platycodin D contained in a Platycodon grandiflorum extract as well as an extract of Platycodon grandiflorum, Scutellaria baicalensis, Phellodendron amurense Ruprecht, or Rubus coreanus or a fraction thereof can be used for the prevention, improvement, or treatment of GBM.

Problems solved by technology

However, in the case of temozolomide, the treatment is only effective for some patients and the average survival rate of GBM patients by the treatment is only 14 months, and thus, the development of a more effective treatment is imperative.

Method used

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  • Composition for preventing or treating glioblastoma comprising <i>Platycodon grandiflorum </i>A. De Candolle, <i>Scutellaria baicalensis, Phellodendron amurense </i>Ruprecht or <i>Rubus coreanus </i>
  • Composition for preventing or treating glioblastoma comprising <i>Platycodon grandiflorum </i>A. De Candolle, <i>Scutellaria baicalensis, Phellodendron amurense </i>Ruprecht or <i>Rubus coreanus </i>
  • Composition for preventing or treating glioblastoma comprising <i>Platycodon grandiflorum </i>A. De Candolle, <i>Scutellaria baicalensis, Phellodendron amurense </i>Ruprecht or <i>Rubus coreanus </i>

Examples

Experimental program
Comparison scheme
Effect test

example 7

Immunofluorescence Analysis

[0128]Cells treated with the materials were permeabilized with 0.01% Triton X-100 in PBS. After blocking the resulting cells in 2% bovine serum albumin (BSA) in PBS, they were incubated at 4° C. with a primary antibody against SQSTM1 / p62 (Santa Cruz) overnight. The stained cells were incubated with an Alexa594-conjugated secondary antibody (Invitrogen, Calif., USA). The images were examined under a Zeiss laser confocal microscope.

example 8

Lysotracker Assay

[0129]To stain acidic compartments, 50 nM LysoTrackerRedDND-99 (Thermo Fisher Scientific) was added to a medium at 37° C. with 5% CO2 for 30 minutes prior to fixation. The nuclei were labeled with 4,6-diamidino-2-phenylindole (DAPI, Sigma). The slides were finally mounted using a Faramount mounting medium (Dako, Glostrup, Denmark). The images were examined under a Zeiss laser confocal microscope.

example 9

DO Red-BSA Trafficking Assay

[0130]Cells were placed on coverslips prior to treatment with the materials and continuously loaded with DQ Red-BSA (Invitrogen) at a working concentration of 10 μg / mL in a culture medium at 37° C. for 30 minutes. The cells were fixed in 4% PFA and then subjected to DAPI staining. The images were examined under a Zeiss laser confocal microscope. The number of DQ Red-BSA spots was quantified using ImageJ software.

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Abstract

The present invention relates to an extract of at least any one selected from the group consisting of Platycodon grandiflorum, Scutellaria baicalensis, Phellodendron amurense Ruprecht, and Rubus coreanus, or a fraction thereof; and to a use of the same for prevention, improvement, or treatment of glioblastoma multiforme.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority to Korean Application No. 10-2019-0016297, filed on Feb. 12, 2019, and Korean Application No. 10-2019-0045700, filed on Apr. 18, 2019, the contents of both prior applications being hereby incorporated by reference in their entirety.TECHNICAL FIELD[0002]The present invention relates to an extract of at least any one selected from the group consisting of Platycodon grandiflorum, Scutellaria baicalensis, Phellodendron amurense Ruprecht, and Rubus coreanus, or a fraction thereof; and to a use of the same for prevention, improvement, or treatment of glioblastoma multiforme.BACKGROUND ART[0003]Glioblastoma multiforme (hereinafter, GBM) refers to the most malignant glioma (Grade IV by the WHO classification) among primary brain cancers, and it is known to have a very poor prognosis. The standard treatment for patients with GBM includes surgical resection, radiation therapy, and chemotherapy using temozolomide. Ho...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): A61K36/539A61K36/346A61K36/73A61K36/756
CPCA61K36/539A61K36/346A61K36/73A61K36/756A61K2236/331A61K2236/333A61K2300/00
Inventor KO, SEONG-GYU
Owner UNIV IND COOP GRP OF KYUNG HEE UNIV
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