Diagnosis of diseases associated with metastasis

a technology for diagnosis and metastasis, applied in the direction of specific peptides, material analysis, peptide sources, etc., can solve the problems of suppressing tumor cell growth, heritable levels, and the inability to identify the position of 5-methylcytosine by sequencing

Inactive Publication Date: 2003-08-07
EPIGENOMICS AG
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Problems solved by technology

This resulted in heritable levels of gene expression leading to suppression of growth in tumor cell lines.
However, 5-methylcytosine positions cannot be identified by sequencing since 5-methylcytosine has the same base pairing behavior as cytosine.
Moreover, the epigenetic information carried by 5-methylcytosine is completely lost during PCR amplification.
However, 5-methylcytosine remains unmodified under these conditions.
However, currently only individual regions of a length of up to approximately 3000 base pairs are analyzed, a global analysis of cells for thousands of possible methylation events is not possible.
However, this method cannot reliably analyze very small fragments from small sample quantities either.
These are lost through the matrix in spite of the diffusion protection.
For nucleic acids having a multiply negatively charged backbone, the ionization process via the matrix is considerably less efficient.

Method used

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Examples

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Diagnosis of diseases associated with metastasis

[0068] In order to relate the methylation patterns to one of the diseases associated with metastasis, it is initially required to analyze the DNA methylation patterns of a group of diseased and of a group of healthy patients. These analyses are carried out, for example, analogously to Example 1. The results obtained in this manner are stored in a database and the CpG dinucleotides which are methylated differently between the two groups are identified. This can be carried out by determining individual CpG methylation rates as can be done, for example, in a relatively imprecise manner, by sequencing or else, in a very precise manner, by a methylation-sensitive "primer extension reaction". It is also possible for the entire methylation status to be analyzed simultaneously, and for the patterns to be compared, for example, by clustering analyses which can be carried out, for example, by a computer.

[0069] Subsequently, it is possible to all...

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Abstract

The present invention relates to the chemically modified genomic sequences of genes associated with metastasis, to oligonucleotides and / or PNA-oligomers for detecting the cytosine methylation state of genes associated with metastasis which are directed against the sequence, as well as to a method for ascertaining genetic and / or epigenetic parameters of genes associated with metastasis.

Description

[0001] The levels of observation that have been well studied by the methodological developments of recent years in molecular biology, are the genes themselves, the translation of these genes into RNA, and the resulting proteins. The question of which gene is switched on at which point in the course of the development of an individual, and how the activation and inhibition of specific genes in specific cells and tissues are controlled is correlatable to the degree and character of the methylation of the genes or of the genome. In this respect, pathogenic conditions may manifest themselves in a changed methylation pattern of individual genes or of the genome.[0002] The present invention relates to nucleic acids, oligonucleotides, PNA-oligomers and to a method for the diagnosis and / or therapy of diseases which have a connection with the genetic and / or epigenetic parameters of genes associated with metastasis and, in particular, with the methylation status thereof.PRIOR ART[0003] The ke...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N27/62A61K31/711A61K48/00A61P7/04A61P9/10A61P11/06A61P13/12A61P29/00A61P35/00B01J19/00C07K14/46C07K14/47C07K14/82C12M1/00C12N15/09C12Q1/48C12Q1/68G01N33/483G01N33/53G01N33/566G01N37/00
CPCC07K14/4703C07K14/82C12Q2600/156C12Q1/6886C12Q2600/154C12Q1/6883A61P11/06A61P13/12A61P29/00A61P35/00A61P7/04A61P9/10
Inventor OLEK, ALEXANDERPIEPENBROCK, CHRISTIANBERLIN, KURT
Owner EPIGENOMICS AG
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