Diagnosis of diseases associated with metastasis
a technology for diagnosis and metastasis, applied in the direction of specific peptides, material analysis, peptide sources, etc., can solve the problems of suppressing tumor cell growth, heritable levels, and the inability to identify the position of 5-methylcytosine by sequencing
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Diagnosis of diseases associated with metastasis
[0068] In order to relate the methylation patterns to one of the diseases associated with metastasis, it is initially required to analyze the DNA methylation patterns of a group of diseased and of a group of healthy patients. These analyses are carried out, for example, analogously to Example 1. The results obtained in this manner are stored in a database and the CpG dinucleotides which are methylated differently between the two groups are identified. This can be carried out by determining individual CpG methylation rates as can be done, for example, in a relatively imprecise manner, by sequencing or else, in a very precise manner, by a methylation-sensitive "primer extension reaction". It is also possible for the entire methylation status to be analyzed simultaneously, and for the patterns to be compared, for example, by clustering analyses which can be carried out, for example, by a computer.
[0069] Subsequently, it is possible to all...
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