Bioresorbable and biocompatible compounds for surgical use

a biocompatible and surgical technology, applied in the field of biocompatible and bioresorbable compounds, can solve the problems of inconvenient use of cross linker agents such as glutaraldehyde in certain applications, insufficient in-vivo biodegradation of collagen, and difficult manipulation of collagen and glycosaminoglycans,

Inactive Publication Date: 2009-03-12
SOFRADIM PROD SAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-crosslinked collagen and chitosan mixtures have weak mechanical properties rendering their manipulation difficult and the in-vivo biodegradation of the collagen is often insufficient.
Cross linking of collagen and glycosaminoglycans, e.g. chitosan, using a cross linker agent such as glutaraldehyde is inconvenient in certain applications.
For example, the use of glutaraldehyde in aqueous media leads to the formation of very high molecular glutaraldehyde polymers which are difficult to eliminate by simple washing techniques.

Method used

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  • Bioresorbable and biocompatible compounds for surgical use
  • Bioresorbable and biocompatible compounds for surgical use
  • Bioresorbable and biocompatible compounds for surgical use

Examples

Experimental program
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Effect test

example 1

Freeze Dried Sponge for the Preparation of Materials Supporting Cell Growth

[0053]A collagen / chitosan mixture was prepared by mixing an acidic solution of oxidized collagen and an acidic solution of chitosan in different proportions with a final polymer concentration of 1% (w / w).

Oxidized Collagen

[0054]Oxidized collagen was obtained by the oxidation of a 3% collagen solution by periodic acid, at a final concentration of 8 mM, at room temperature, during 3 hours, as described by Bayon, et al. in Example 4 of U.S. Pat. No. 6,596,304. At this step the pH of the oxidized collagen solution was about 3.2.

Native Collagen

[0055]Solutions of native collagen were obtained by solubilizing collagen powder at a 1% final concentration, in sterile water. The pH measured close to 3.

Chitosan

[0056]The chitosan was solubilized in deionized water with a stoechiometric amount of hydrochloric acid with a polymer concentration of 1% (w / w). The pH of the chitosan solution was about 5, but the pH could have be...

example 2

Preparation of Cylindrical Structures for Supporting Nervous Cells Growth

[0066]A collagen / chitosan mixture was prepared by mixing an acidic solution of oxidized collagen and acidic solution of chitosan in different proportions as described above in Example 1, with a final polymer concentration of 2% (w / w).

[0067]The mixture was poured into cylindrical moulds of different diameters ranging from 1 mm to 10 mm and freeze-dried for about 24 hours.

[0068]Thereafter, the cylinders were neutralized in a buffer solution of PBS 1× for about 2 hours and then dried in a ventilated oven at 35° C. overnight.

example 3

Preparation of Tubular Structures for Supporting Endothelial Cells Growth

[0069]A collagen / chitosan mixture was prepared by mixing an acidic solution of oxidized collagen and acidic solution of chitosan in different proportions as described above in Example 1, with a final polymer concentration of 2% (w / w).

[0070]The mixture (about 40 g) was poured into tubular moulds of different diameters ranging from 5 mm to 15 mm and freeze-dried for 24 hours.

[0071]Thereafter, the tubes were neutralized in a buffer solution of PBS 1× for 2 hours and then dried in a ventilated oven at 35° C. overnight.

[0072]Optionally, a 20 / 80 mixture of the oxidized collagen and chitosan with a final polymer concentration of 0.5% (w / w) with a pH adjusted to 5 was used to coat the external surface of the tubular structure bringing different permeability properties to the tubular composite material.

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Abstract

A bioresorbable and biocompatible compound for surgical use is composed of functionalized collagen cross-linked with a glycosaminoglycan.

Description

TECHNICAL FIELD[0001]The present disclosure relates to biocompatible and bioresorbable compounds containing a functionalized collagen covalently bonded directly to a glycosaminoglycan without the use of a chemical cross-linking agent. The present compounds and compositions containing them are useful for a variety of medical applications, including surgical implants.DESCRIPTION OF THE RELATED ART[0002]Collagen and glycosaminoglycans have been combined for the preparation of biomaterials and surgical implants. Non-crosslinked collagen and chitosan mixtures have weak mechanical properties rendering their manipulation difficult and the in-vivo biodegradation of the collagen is often insufficient.[0003]Cross linking of collagen and glycosaminoglycans, e.g. chitosan, using a cross linker agent such as glutaraldehyde is inconvenient in certain applications. For example, the use of glutaraldehyde in aqueous media leads to the formation of very high molecular glutaraldehyde polymers which ar...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61F2/00A61K31/726A61K38/36C07K14/78
CPCA61L27/24A61L27/20
Inventor GRAVAGNA, PHILIPPEBAYON, YVEZLADET, SEBASTIEN
Owner SOFRADIM PROD SAS
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