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Extract of Polygonum multiflorum Thunb. ex Murray var. hypoleucum and compositions for improving metabolic syndrome

a technology of polygonum multiflorum and which is applied in the field of extract of polygonum multiflorum thunb. ex murray var. hypoleucum for improving metabolic syndrome, can solve the problems of not being able not being able to modulate blood glucose level, and foregoing patents not being able to disclose how to improve metabolic syndrome, etc., to achieve the effect of stimulating acyl-

Inactive Publication Date: 2009-12-10
FOOD IND RES & DEV INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0040]The extract of Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi), or of Polygonum hypoleucum (Ohwi) of the present invention is prepared easily and can modulate blood glucose and blood lipid levels by inhibiting the activities of glucosidic ...

Problems solved by technology

However, the foregoing prior arts are not capable of use for modulating the blood lipid, nor improving metabolic syndrome.
In addition, the foregoing patent did not disclose how to modulate blood glucose level nor to improve metabolic syndrome.

Method used

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  • Extract of Polygonum multiflorum Thunb. ex Murray var. hypoleucum and compositions for improving metabolic syndrome
  • Extract of Polygonum multiflorum Thunb. ex Murray var. hypoleucum and compositions for improving metabolic syndrome
  • Extract of Polygonum multiflorum Thunb. ex Murray var. hypoleucum and compositions for improving metabolic syndrome

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Extracts of Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi) or of Polygonum hypoleucum (Ohwi)

[0055]i) Acidic Alcohol Extract

[0056]Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi) or Polygonum hypoleucum (Ohwi) was machine-grounded, then extracted with an acidic alcohol containing 0.1% HC1. The weight to volume ratio of grounded Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi) or Polygonum hypoleucum (Ohwi)and acidic alcohol was about 1:8. The mix was left at room temperature with or without shaking for 72 hours, filtered through 2 layers of cheesecloth and centrifuged at 6,000 rpm for 15 min. The solution was collected and concentrated. The extraction rate is about 6.9%.

[0057]A 50 mg / ml stock solution was prepared with water.

[0058]ii) Hot Water Extract:

[0059]Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi) or Polygonum hypoleucum (Ohwi) was heated in water for 2 hours. The weight to volume ratio of Polygonum multifl...

example 2

The Inhibitory Effects of Extracts of Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi) or of Polygonum hypoleucum (Ohwi)on α-glucosidase

[0060]Extract #1 and #2 are different batches of acidic alcohol extracts, and extract #3 is hot water extract, prepared as described in example 1.

[0061]The inhibitory effects of the 3 extracts on α-glucosidase was assayed as following:

[0062]1. Both enzyme α-glucosidase (EC 3.2.1.20, G5003, Sigma-Aldrich Co., USA) and substrate p-nitrophenyl α-D-glucopyranoside (pNP-G) (Sigma-Aldrich Co., USA) solutions were prepared with 100 mM, pH 7.0 phosphoric acid buffer solution to reached a concentration of 1 U / mland 5×10−3 M, respectively.

[0063]2. 40 μl of 0.4 M, pH 7.0 phosphoric acid buffer, 10 μl of α-glucosidase, 50 μl of serial dilutions of the extracts of Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi) or of Polygonum hypoleucum (Ohwi)and 170 μl of deionized water were added orderly into each well of a 96-well plate

[0064]3. An...

example 3

The Inhibitory Effects of Extracts of Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi) or of Polygonum hypoleucum (Ohwi) on Different Glycosidic Enzymes

[0067]Rat intestinal acetone powder (Sigma Chemical Co., St. Louis, Mo.) was provided as a source of glycosidic enzyme to test the inhibitory effects of the acidic alcohol extracts with various glycosidic substrates (maltose, starch and sucrose)(Table 2).

TABLE 2Values of IC50 of the acidic alcohol extract of Polygonummultiflorum Thunb. ex Murray var. hypoleucum (Ohwi) for variousglycosidic enzymesIC50(mg / ml)Maltaseα-amylaseSucraseAcidic alcohol extract0.61.93.1

[0068]Table 2. Values of IC50 of the acidic alcohol extract of Polygonum multifloum Thunb. ex Murray var. hypoleucum (Ohwi) for various glycosidic enzymes

[0069]As shown in Table 2, the acidic alcohol extract was capable of inhibiting the activities of maltase, α-amylase and sucrase, with the strongest effects on maltase.

EXAMLE 4

Preparation of Lipid-Soluble Extracts...

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Abstract

An extract of Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi) or of Polygonum hypoleucum (Ohwi) for improving metabolic syndrome is prepared by the following method: (a) providing fresh or dry Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi) or Polygonum hypoleucum (Ohwi) in partial or whole plant ; (b) extracting the partial or whole plant by a solvent to obtain a crude extract; (c) condensing and drying the crude extract to obtain an extract product; and (d) collecting the extract product. The present invention also relates to a pharmaceutical and a food composition having the above extract for improving the metabolic syndromes. The extract of the present invention can be easily prepared and is demonstrated to modulate the levels of both blood glucose and blood lipid, to inhibit the activities of glycolytic enzymes and acetyl-CoA carboxylase, and to stimulate acyl-CoA oxidase. The extract of the present invention is capable of improving metabolic syndromes.

Description

BACKGROLND OF THE MWNTIQN[0001]1. Field of the Invention[0002]The present invention relates to an extract of Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi), especially to an extract of Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi) for improving metabolic syndrome.[0003]2. Description of the Related Art[0004]Metabolic syndrome is a combination of medical disorders that increase a person's risk of developing cardiovascular diseases and diabetes. Metabolic syndrome affects a large number of people in a clustered fashion. Recently, with changing dietary habits and lifestyle, more and more people are being diagnosed as having metabolic syndrome that have symptoms including high blood glucose, high blood pressure or high blood lipid level.[0005]Metabolic syndrome may specifically refer to insulin resistance syndrome.[0006]Polygonum multiflorum Thunb. ex Murray var. hypoleucum (Ohwi), or Polygonum hypoleucum (Ohwi), is a special, endemic variety found in Taiw...

Claims

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Application Information

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IPC IPC(8): A61K36/00A61P3/10
CPCA61K36/704A61P3/00A61P3/10
Inventor CHEN, CHI-HUACHEN, SHIOW-WENCHANG, YUARN-YEELIU, WEN-KIA
Owner FOOD IND RES & DEV INST
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