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Yield and stress tolerance in transgenic plants iii

a transgenic plant and stress tolerance technology, applied in the field of plant genomics and plant improvement, can solve the problems of not providing one with a means to determine, insufficient homology identification, etc., and achieve the effect of enhancing the yield of commercially significant plants and ameliorating the adverse effects of water or nutrient defici

Inactive Publication Date: 2010-07-22
MONSANTO CO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0010]An object of this invention is to provide plants which can express genes to increase yield of commercially significant plants, as well as to ameliorate the adverse effects of water or nutrient deficit.
[0023]The expression vectors, and hence the transgenic plants, of the invention, comprise putative transcription factor polynucleotides sequences and, in particular, B-box zinc finger sequences. When any of these polypeptide of the invention is overexpressed in a plant, the polypeptide confers at least one regulatory activity to the plant, which in turn in manifested in a trait selected from the group consisting of increased yield, greater height, increased secondary rooting, greater cold tolerance, greater tolerance to water deprivation, reducing stomatal conductance, altered C / N sensing, increased low nitrogen tolerance, increased low phosphorus tolerance, and increased tolerance to hyperosmotic stress as compared to the control plant.

Problems solved by technology

However, in many cases, the identification of homologs is not sufficient to make specific predictions because not all homologs have the same function.
Thus, an initial analysis of functional relatedness based on sequence similarity alone may not provide one with a means to determine where similarity ends and functional relatedness begins.

Method used

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  • Yield and stress tolerance in transgenic plants iii
  • Yield and stress tolerance in transgenic plants iii
  • Yield and stress tolerance in transgenic plants iii

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examples

[0171]It is to be understood that this invention is not limited to the particular devices, machines, materials and methods described. Although particular embodiments are described, equivalent embodiments may be used to practice the invention.

[0172]The invention, now being generally described, will be more readily understood by reference to the following examples, which are included merely for purposes of illustration of certain aspects and embodiments of the present invention and are not intended to limit the invention. It will be recognized by one of skill in the art that a polypeptide that is associated with a particular first trait may also be associated with at least one other, unrelated and inherent second trait which was not predicted by the first trait.

example i

Project Types and Vector and Cloning Information

[0173]A number of constructs were used to modulate the activity of sequences of the invention. An individual project was defined as the analysis of lines for a particular construct (for example, this might include G1988 lines that constitutively overexpressed a sequence of the invention). In the present study, a full-length wild-type version of a gene was directly fused to a promoter that drove its expression in transgenic plants. Such a promoter could be the native promoter of that gene, or a constitutive promoter such as the cauliflower mosaic virus 35S promoter. Alternatively, a promoter that drives tissue specific or conditional expression could be used in similar studies.

[0174]In the present study, expression of a given polynucleotide from a particular promoter was achieved by a direct-promoter fusion construct in which that sequence was cloned directly behind the promoter of interest. A direct fusion approach has the advantage of...

example ii

Transformation

[0179]Transformation of Arabidopsis was performed by an Agrobacterium-mediated protocol based on the method of Bechtold and Pelletier (1998). Unless otherwise specified, all experimental work was done using the Columbia ecotype.

[0180]Plant preparation. Arabidopsis seeds were sown on mesh covered pots. The seedlings were thinned so that 6-10 evenly spaced plants remained on each pot 10 days after planting. The primary bolts were cut off a week before transformation to break apical dominance and encourage auxiliary shoots to form. Transformation was typically performed at 4-5 weeks after sowing.

[0181]Bacterial culture preparation. Agrobacterium stocks were inoculated from single colony plates or from glycerol stocks and grown with the appropriate antibiotics and grown until saturation. On the morning of transformation, the saturated cultures were centrifuged and bacterial pellets were re-suspended in Infiltration Media (0.5×MS, 1× B5 Vitamins, 5% sucrose, 1 mg / ml benzyla...

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Abstract

Polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. The polypeptides of the invention have been shown to confer at least one regulatory activity and confer increased yield, greater height, greater early season growth, greater canopy coverage, greater stem diameter, greater late season vigor, increased secondary rooting, more rapid germination, greater cold tolerance, greater tolerance to water deprivation, reduced stomatal conductance, altered C / N sensing, increased low nitrogen tolerance, increased low phosphorus tolerance, or increased tolerance to hyperosmotic stress as compared to the control plant as compared to a control plant.

Description

RELATIONSHIP TO COPENDING APPLICATIONS[0001]This application is a divisional application of prior-filed U.S. patent application Ser. No. 11 / 821,448, filed Jun. 22, 2007 (pending). U.S. patent application Ser. No. 11 / 821,448 claims the benefit of U.S. provisional application 60 / 817,886, filed Jun. 29, 2006 (expired). U.S. patent application Ser. No. 11 / 821,448 is also a continuation-in-part of prior-filed U.S. patent application Ser. No. 11 / 642,814, filed Dec. 20, 2006 (pending), which is a divisional application of prior-filed U.S. patent application Ser. No. 10 / 666,642, filed Sep. 18, 2003, and which issued as U.S. Pat. No. 7,196,245 on Mar. 27, 2007, the latter application claiming the benefit of prior-filed U.S. provisional application 60 / 411,837, filed Sep. 18, 2002 (expired), U.S. provisional application 60 / 434,166, filed Dec. 17, 2002 (expired), and U.S. provisional application 60 / 465,809, filed Apr. 24, 2003 (expired). The entire contents of each of these applications are her...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01H5/00C12N15/82A01H1/06
CPCC07K14/415C12N15/8261C12N15/827C12N15/00C12N15/8262C12N15/8273Y02A40/146C12N15/8271
Inventor CREELMAN, ROBERT A.GUTTERSON, NEAL I.RATCLIFFE, OLIVER J.REUBER, T. LYNNECERNY, R. ERICDUFF, KIMBERLY FAYE ZOBRISTKJEMTRUP-LOVELACE, SUSANNEMEISTER, ROBERT J.PETRACEK, MARIE E.RUFF, THOMASXU, QINGZHANG
Owner MONSANTO CO
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