Nf-kb inhibitor
a technology of nf-kb and inhibitor, which is applied in the field of nf-kb inhibitor, can solve problems such as unclearness, and achieve the effect of convenient consumption and obtained
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example 1
1. Materials
[0033]As Sargassum horneri, Sargassum horneri harvested from the coast at Iwate prefecture was used. The Sargassum horneri was ground, and thereafter purified distilled water was added to the obtained Sargassum horneri ground product. The mixture was subjected to suspension extraction with a Potter homogenizer for 10 to minutes, and the extract was centrifuged using a centrifuge at room temperature (10 to 25° C.) at 3,000 rotations for 30 minutes. After the centrifugation, a supernatant thereof was recovered, and a Sargassum horneri water extract having a molecular weight of 3,000 or less was isolated by membrane filtration. Such a Sargassum horneri water extract was freeze-dried, and dissolved in purified distilled water to be used, when used in an experiment.
example 2
2. Method
2-1 Method of Pre-incubation of Osteoblast
[0034]Osteoblasts were pre-incubated in accordance with the method described in the document (Yamaguchi M, Weitzmann M N: Vitamin K2 stimulates osteoblastogenesis and suppresses osteoclastogenesis by NF-κB activation. Int J Mol Med 27: 3-14 (2011)). That is to say, 1 ml of α-MEM (α-modified essential medium) (manufactured by Invitrogen Corporation) culture solution containing 10% fetal bovine serum (FBS) (hereinafter simply referred to as culture solution) was added to a 12-well culture plate, osteoblasts (the number of cells: 1×105 cells / ml / well) were transferred into the culture solution, and pre-incubation was carried out at 37° C. in a CO2 incubator for 3 days.
2-2 Method of Calcification (Mineralization) Analysis of Osteoblast Using Sargassum horneri Water Extract
[0035]Calcification analysis of osteoblasts using the Sargassum horneri water extract was carried out in accordance with the procedure shown in the following [1] to [8]...
example 3
3. Results and Discussion
[0040]3-1 NF-κB Activation Inhibitory Effect of Sargassum horneri Water Extract of the Present Invention in Osteoblast
[0041]TNF-α regulates diverse cellular functions via NF-κB activation signal in the cell, and, for example, in osteoblasts, it is known that TNF-α suppresses calcification of osteoblasts (Non-patent Documents 4, 5, Yamaguchi M, Weitzmann M N: Vitamin K2 stimulates osteoblastogenesis and suppresses osteoclastogenesis by NF-κB activation. Int J Mol Med 27: 3-14 (2011), Li Y, Li A, Strait K, Zhang H, Nanes M S and Weitzmann M N: Endogenous TNF alpha Lowers Maximum Peak Bone Mass and Inhibits Osteoblastic Smad Activation, through NF-kappa B. Bone Miner Res 22: 646-655 (2007)). Also in the present experiment, it was confirmed that, when osteoblasts were cultured after TNF-α (1 ng / ml culture solution) was added, calcification of the osteoblasts was inhibited. It was revealed that such calcification inhibition by TNF-α in osteoblasts is significantl...
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