Refined plant isolates and process for the manufacture of a functional food ingredient from such plant isolate

a technology of plant isolates and refined plant isolates, which is applied in the field of refined plant isolates and process for the manufacture of functional food ingredients from such plant isolates, can solve the problems of increasing chromatographic resolution of charged and neutral compounds, affecting the separation of charged compounds, and reducing so as to reduce the number of available charges and impair the ion exclusion ability of the resin bed.

a technology of plant isolates and refined plant isolates, which is applied in the field of refined plant isolates and process for the manufacture of functional food ingredients from such plant isolates, can solve the problems of increasing chromatographic resolution of charged and neutral compounds, affecting the separation of charged compounds, and reducing so as to reduce the number of available charges and impair the ion exclusion ability of the resin bed.

US20130259987A1Inactive Publication Date: 2013-10-03CONOPCO INC D B A UNILEVER
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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0111]A concentrated permeate (40 Brix) was prepared in the same way as described in the Comparative Example. The concentrated permeate was contacted with ion exchange resin to remove Ca and Mg prior to being used as a feed for ion exclusion chromatography.

[0112]Production of Refined Tomato Isolate

[0113]The ion exchange, IEX, step was performed in a stirred vessel configuration. The concentrated permeate was mixed in a vessel with the ion-exchanger (Lewatitm 2568 resin) with the help of a top agitator. 1.5 Liter of resin was added in a vessel and preconditioned with a number of washing steps for removing all other minerals and bringing the resin in the K form. First the resin was acid washed by addition of 3 Liters of 10% w / w HCL that remained in contact under vigorous stirring for 1 hr. The acid was removed by washing steps with demineralised water until the conductivity of the decanted effluents was lower than 20 microS / cm. An alkaline washing step of the resin took place by addit...

example 2

[0119]A concentrated permeate (40 Brix) was prepared in the same way as described in the Comparative Example. The concentrated permeate was subjected to ion exchange chromatography to remove Ca and Mg prior to being used as a feed for ion exclusion chromatography.

[0120]Production of Refined Tomato Isolate

[0121]Ion exchange chromatography was performed on a 4 column pilot scale ion exchanger apparatus manufactured by UNIPEKTIN™ Engineering AG, Eschenz, Switzerland. The bed volume of each column was 75 liters with an inner diameter of 20 cm. A 2×2 column configuration was used. The feed followed by the demi water eluent were pumped by the main pump and were divided into 2 streams. Each stream passed via 2 columns connected in series and was then collected at the exit of the ion exchange unit. The flow was adjusted by means of magnetic inductive flow meters.

[0122]Lewatitm 2568 resin was mixed with water and packed in the columns. Next, a number of washing steps were used to bring the r...

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Abstract

The present invention is concerned with providing refined plant isolates that can be used as starting materials for the production of functional food ingredients, such as taste enhancers, through an isolation process that employs ion exclusion chromatography. More particularly, the present invention provides a refined plant isolate comprising at least 30% by weight of dry matter of water soluble plant components selected from fructose, glucose, proteinaceous matter of plant origin, acids and potassium, said isolate further being characterized in that at a concentration of 40° Brix the isolate: •contains at least 0.3 wt % of glutamate; •contains at least 500 ppm of K+; •contains less than 300 ppm of Ca2+; •contains less than 700 ppm of Mg •contains less than 1 wt. % of components capable of passing an ultra filtration membrane having a molecular weight cut off of 250 kDa; wherein the concentration ratio ([Ca2+]+[Mg2+]) / [K−] does not exceed 5:100, each of said cation concentrations being expressed in ppm.

Description

TECHNICAL FIELD OF THE INVENTION[0001]The present invention relates to the isolation of functional food ingredients from plant materials that are rich in glutamate and potassium, such as tomatoes, potatoes, peas, corn, beets, carrots, onions, spinach, green peppers etc. More particularly, the present invention is concerned with providing refined plant isolates that can advantageously be used as starting materials for the production of functional food ingredients, such as taste enhancers, through an isolation process that employs ion exclusion chromatography. The invention further provides a process for the manufacture of functional food ingredients from the aforementioned refined plant isolates.BACKGROUND OF THE INVENTION[0002]Plant materials are an economic source of a number widely utilized functional food ingredients, notably biopolymers. Thus, it is well-known in the art to isolate functional food ingredients such as starch, inulin, pectin and proteins, from plant materials.[000...

Claims

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Application Information

Patent Timeline
03 Oct 2013
Publication
US20130259987A1
IPC
A23L1/30; A23L5/20; A23L19/00; A23L27/00; A23L27/10; A23L27/12; A23L27/22
CPC
A23L1/0156; A23L1/2128; A23L1/221; A23L1/222; A23L1/228; A23L2/082; A23L1/304; A23L1/3051
Inventors
MAVROUDIS, NIKOLAOS; MAZZOTTI, MARCO GIUSEPPE