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Lung cancer signature

a lung cancer and signature technology, applied in the field of compositions and methods of cancer diagnosis, research and therapy, can solve the problem of not being able to identify which specific patients are at high risk of relaps

Inactive Publication Date: 2015-06-04
RGT UNIV OF MICHIGAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides compositions and methods for diagnosing and treating lung cancer by using a panel of cancer markers. These markers can be detected using nucleic acid probes, amplification primers, sequencing primers, or antibodies. The invention also provides a kit for determining the likelihood of survival, metastasis, or advancement of lung cancer in a subject. The use of these markers can help improve the accuracy of diagnosis and treatment of lung cancer.

Problems solved by technology

While it is known that 35-50% of patients with stage I disease will relapse within five years (Williams et al., Thorac. Cardiovasc. Surg. 82:70 ; Pairolero et al., Ann. Thorac. Surg. 38:331 ), it is not currently possible to identify which specific patients are at high risk of relapse.

Method used

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Examples

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Methods

[0092]Cell culture: The A549 human lung adenocarcinoma cell line was obtained from the American type Culture Collection (Manassas, Va.) and maintained in RPMI-1640 medium with glutamine, supplemented with 10% FBS, penicillin, and streptomycin and tested for mycoplasma contamination. All tissue culture media and media supplements were purchased from Life Technologies (Gaithersburg, Md.). The porcine transforming growth factor beta 1 (TGF-β) was purchased from PeproTech (Rocky Hill, N.J.). In all experiments cells at 40-50% confluency were serum starved for 24 h and treated with TGF-β (5 ng / ml) for 72 h. At the end conditioned media collected was centrifuged at 2000 g for 20 minutes and filtered through 0.2 μm filter to remove the intact cells and debris and stored at −80 0 C until further processing. Cells in the culture dishes were lysed in RIPA buffer and processed for western immunoblotting for assessing the expression of epithelial and mesenchymal markers. Protein concentr...

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Abstract

The present disclosure relates to compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present disclosure relates to cancer markers as diagnostic markers and clinical targets for lung cancer.

Description

[0001]The present application claims priority to U.S. Provisional Patent Application Ser. No. 61 / 904,711, filed Nov. 15, 2013, the disclosure of which is herein incorporated by reference in its entirety.FIELD OF THE INVENTION[0002]The present disclosure relates to compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present disclosure relates to cancer markers as diagnostic markers and clinical targets for lung cancer.BACKGROUND OF THE INVENTION[0003]Lung cancer remains the leading cause of cancer death in industrialized countries. About 75 percent of lung cancer cases are categorized as non-small cell lung cancer (e.g., adenocarcinomas), and the other 25 percent are small cell lung cancer. Lung cancers are characterized in to several stages, based on the spread of the disease. In stage I cancer, the tumor is only in the lung and surrounded by normal tissue. In stage II cancer, cancer has spread to near...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/574C12Q1/68
CPCG01N33/57423C12Q1/6886G01N2800/52C12Q2600/118C12Q2600/106C12Q2600/158
Inventor KESHAMOUNI, VENKATESHWAR G.OMENN, GILBERT S.CHEN, GUOANBEER, DAVID G.STANDIFORD, THEODORE J.
Owner RGT UNIV OF MICHIGAN
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