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Creation of libraries of droplets and related species

a technology of droplets and related species, which is applied in the preparation of microcapsules, biochemistry apparatus and processes, chemical/physical/physico-chemical processes, etc., can solve the problems of cost, time, equipment, etc., that affect the preparation, storage and use of current technologies

Inactive Publication Date: 2015-11-26
PRESIDENT & FELLOWS OF HARVARD COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach enables the efficient production and manipulation of droplets containing various species, facilitating the formation of libraries that can be used for screening and analysis, such as nucleic acid sequencing, while reducing costs and time associated with existing technologies.

Problems solved by technology

Although current libraries are valuable, commercially-available resources useful in the search to identify new compounds with desirable activities, there are practical difficulties (e.g., cost, time, equipment, etc.) associated with the preparation, storage, and use of current technologies.

Method used

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  • Creation of libraries of droplets and related species
  • Creation of libraries of droplets and related species
  • Creation of libraries of droplets and related species

Examples

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example 1

[0126]The following example describes the formation of a plurality of rigidified droplets comprising a first group of rigidified droplets comprising a first species and a second group of rigidified droplets comprising a second species, wherein the first species and the second species are distinguishable from each other, according to one embodiment of the present invention.

[0127]A plurality of rigidified droplets were formed using microfluidic techniques as follows. A solution comprising water, 5% acrylamide, 0.25% bisacrylamide, 0.1% ammonium persulfate, and 1 uM of a first acrydite-labeled DNA oligonucleotide 20-mer was flowed through the center channel of a microfluidic device and focused by oil containing 0.1% TEMED in the side channels through a 10 micron nozzle to produce approximately 12 micron pre-gel droplets, as depicted in FIG. 9A. The droplets were allowed to rigidify for 30 minutes at 50° C. via polymerization reactions. FIG. 9B shows the plurality of rigidified droplets...

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Abstract

The present invention is generally related to systems and methods for producing a plurality of droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, the fluidic droplets may be rigidified to form rigidified droplets (e.g., gel droplets). In certain embodiments, the droplets may undergo a phase change (e.g., from rigidified droplets to fluidized droplets), as discussed more herein. In some cases, a species may be added internally to a droplet by exposing the droplet to a fluid comprising a plurality of species.

Description

RELATED APPLICATIONS[0001]This application is a continuation of Ser. No. 13 / 119,470, with a filing date of May 4, 2011 which is a national stage filing under 35 U.S.C. §371 of International Application Serial No. PCT / US2009 / 005184, filed Sep. 17, 2009, which claims the benefit of U.S. Provisional Patent Application Ser. No. 61 / 098,674, filed Sep. 19, 2008, entitled “Creation of Libraries of Droplets and Related Species,” by Weitz, et al., herein incorporated by reference.GOVERNMENT FUNDING[0002]This invention was made with government support under DMR-0602684 awarded by the National Institutes of Health. The U.S. government has certain rights in the invention.FIELD OF INVENTION[0003]The present invention is generally related to systems and methods for producing a plurality of droplets. The droplets may contain varying species, e.g., for use as a library. The droplets may be rigidified and / or fluidized, in some cases.BACKGROUND[0004]Various technologies have been developed that allow...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): B01J19/00C12Q1/68
CPCB01J19/0046C12Q1/6874B01J2219/00722B01J2219/00709B01J2219/00599B01J13/04B01J2219/00716B01J2219/00711C12Q1/6876
Inventor WEITZ, DAVID A.AGRESTI, JEREMY
Owner PRESIDENT & FELLOWS OF HARVARD COLLEGE