Application of metrnl protein in preparing hypolipidemic, hypoglycemic medicine
a technology of metrnl protein and lipidemic medicine, applied in the field of medicine, can solve the problems of chronic damage and dysfunction of various tissues, eye, kidney, heart, etc., and achieve the effects of preventing the increase of blood glucose, preventing the increase of blood lipids, and little side effects
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embodiment 1
Preparation of Metrnl Adipose-Specific Overexpression Mice
[0035]A plasmid containing Fabp4-Metrnl was constructed with the promoter region of Fabp4 and open reading frame of Metrnl. After the verification with sequencing and linearization with restriction enzyme cutting, this plasmid was injected into fertilized eggs which were transplanted into the uterus of pseudopregnant mice. After genomic identification of the offspring, the mice carrying the Fabp4-Metrnl in genome were chosen to mate with C57BL / 6 mice. The next-generation mice carrying the Fabp4-Metrnl in genome were selected into experimental group, and the mice without Fabp4-Metrnl in genome were selected into control group.
[0036]Plasmid containing Fabp4-Metrnl can also be produced using purchased commodities, for example, open reading frame of Metrnl can be purchased from Thermo (Lot. MMM1013-202763251), and the promoter region of Fabp4 can be obtained from Addgene (No. 11424).
[0037]The Metrnl adipose-specific overexpressio...
embodiment 2
Metrnl Overexpression can Prevent the Increase of Blood Glucose Induced by Intraperitoneal Injection of Glucose
[0038]Mice aged 22 weeks have been fed for 16 weeks with high fat diet (Research Diets, Lot. D12492). Both control wild type and Metrnl adipose-specific overexpression mice were administrated with the same dose of glucose via intraperitoneal injection. Follows are the details.
[0039]Mice aged 22 weeks fasted for 8 hours, and were intraperitoneally injected with glucose at a dose of 1 g / kg. Then, blood glucose was detected at different time points (Table 1) with a one touch ultra glucometer (Johnson & Johnson) via tail vein bleeding.
[0040]The results showed that the blood glucose was dramatically lower in Metrnl adipose-specific overexpression mice than that in control wild type mice (Table 1 and FIG. 1).
TABLE 1Adipose-specific overexpression of Metrnl reduces the basal level of bloodglucose and inhibits the rise of blood glucose induced by intraperitoneal injection ofglucose...
embodiment 3
Metrnl Overexpression can Prevent Hyperlipemia Induced by Gavage with Lipid
[0041]Both control wild type mice and Metrnl adipose-specific overexpression mice aged 12 weeks were administrated with triglyceride by gavage. Follows are the details.
[0042]12-week-old mice fasted for 3 hours, and were administrated with fat emulsion (Lipofundin, B. Braun Melsungen) at a dose of 10 μl / g. Blood samples were obtained from the tail vein at different time points. And 10 μl serum was used to detect the concentration of triglyceride with Triglyceride Colorimetric Assay Kit (E1003, Applygen Technologies Inc.)
[0043]The results showed that the blood triglyceride was significantly lower in Metrnl adipose-specific overexpression mice than that in control wild type mice (Table 2 and FIG. 2).
TABLE 2Adipose-specific overexpression of Metrnl reduces the basal levelof blood triglyceride and prevents the increase of blood triglycerideinduced by gavage with fat emulsionReductionof bloodtriglyceride inSerum tr...
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