Antifungal paenibacillus strains, fusaricidin-type compounds, and their use

a technology of fusaricidin and antifungal paenibacillus, which is applied in the field of new isolated bacterial strains, can solve the problems of variable efficacy and slow speed

Inactive Publication Date: 2017-08-10
BASF AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biopesticides under certain conditions can also have disadvantages, such as high specificity (requiring an exact identification of the pest / pathogen and the use of multiple products), slow speed of action (thus making them unsuitable if a pest outbreak is an immediate threat to a crop), variable efficacy due to the influences of various biotic and abiotic factors (since biopesticides are usually living organisms, which bring about pest / pathogen control by multiplying within the target insect pest / pathogen), and resistance development.

Method used

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  • Antifungal paenibacillus strains, fusaricidin-type compounds, and their use
  • Antifungal paenibacillus strains, fusaricidin-type compounds, and their use
  • Antifungal paenibacillus strains, fusaricidin-type compounds, and their use

Examples

Experimental program
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example 1

of Novel Bacterial Strains of the Invention

[0382]Soil samples from a variety of European locations including Germany were collected. By applying commonly known microbial isolation procedures to these soils, the inventors obtained a variety of bacteria that were further subjected to conventional isolation techniques for providing pure isolates as described herein.

[0383]Standard microbial enrichment technique (C. A. Reddy, T. J. Beveridge, J. A. Breznak, G. A. Marzluf, T. M. Schmidt, and L. R. Snyder (eds.). Methods for General and Molecular Microbiology, Am. Soc. Microbiol., Washington, District of Columbia) was followed to isolate each type of bacteria.

[0384]The following strains have been isolated and deposited under Budapest Treaty with the Deutsche Sammlung von Mikroorganismen und Zellkuturen (DSMZ) on Feb. 20, 2013: a) Lu16774 as deposited with DSMZ having the deposit number DSM 26969[0385]b) Lu17007 as deposited with DSMZ having the deposit number DSM 26970[0386]c) Lu17015 as d...

example 2

ization of Novel Bacterial Strains

example 2.1

quencing

[0387]The 16S rRNA gene sequences of the Paenibacillus strains were determined by direct sequencing of PCR-amplified 16S rDNA at the DSMZ, Braunschweig, Germany.

[0388]Genomic DNA extraction was carried out using the MasterPure™ Gram Positive DNA Purification Kit from Epicentre Biotechnologies according to the manufacturer's instructions. PCR-mediated amplification of the 16S rDNA and purification of the PCR product was carried out as described previously (Int. J. Syst. Bacteriol. 46, 1088-1092, 1996). Purified PCR products were sequenced using the BigDye® Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems) as directed in the manufacturer's protocol. Sequence reactions were electrophoresed using the 3500xL Genetic Analyzer from Applied Biosystems. Sequence ambiguities may be due to the existence of several cistrons encoding 16S rRNAs with different sequences within a single genome (J. Bacteriol. 178(19), 5636-5643, 1996).

[0389]The resulting sequence data from the strain...

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Abstract

The present invention relates to novel isolated bacterial strains, which are members of the genus Paenibacillus, originally isolated from soil and showing antagonistic activity against a broad range of pathogens and being capable of producing antimicrobial metabolites. It was found that the strains Lu16774 and Lu17007 belong to a novel subspecies named Paenibacillus polymyxa ssp. plantarum while the strain Lu17015 belongs to a novel species which is proposed to be Paenibacillus epiphyticus. The present invention also relates to microbial pesticide compositions comprising at least one of such novel bacterial strains, whole culture broth or a cell-free extract or a fraction thereof or at least one metabolite thereof, and/or a mutant of at least one of said novel bacterial strains having all the identifying characteristics of the respective bacterial strain or whole culture broth, cell-free extract, fraction and/or metabolite of the mutant thereof showing antagonistic activity against plant pathogens. The present invention also relates to a method of controlling or suppressing plant pathogens or preventing plant pathogen infections by applying such composition. The present invention also relates to novel fusaricidin-type compounds which are metabolites produced by the strains of the present invention.

Description

FIELD OF THE INVENTION[0001]The present invention relates to novel isolated bacterial strains, which are members of the genus Paenibacillus, originally isolated from soil and showing antagonistic activity against a broad range of pathogens and being capable of producing antimicrobial metabolites. The present invention also relates to microbial pesticide compositions comprising at least one of such novel bacterial strains, whole culture broth or a cell-free extract or a fraction thereof or at least one metabolite thereof, and / or a mutant of at least one of said novel bacterial strains having all the identifying characteristics of the respective bacterial strain or whole culture broth, cell-free extract, fraction and / or metabolite of the mutant thereof showing antagonistic activity against plant pathogens. The present invention also relates to a method of controlling or suppressing plant pathogens or preventing plant pathogen infections by applying such composition. The present invent...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N63/02C07K14/195A01N63/25
CPCC07K14/195A01N63/02A01N63/00C07K7/06C12N1/20A01N63/25C07K7/64C07K7/54C12R2001/01C12N1/205A01N63/10
Inventor SIEPE, ISABELLABRUSER, HEIKEKLAPPACH, KRISTINSCHNEIDER, KARL-HEINRICHSPROTE, PETRAHAGE, KERSTINBLANZ, BIRGITTHINES, ECKHARDANTELO, LUISSANDJO, LOUIS PERGAUDOPATZ, TILL
Owner BASF AG
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