Fc FUSION HIGH AFFINITY IgE RECEPTOR ALPHA-CHAIN
a high affinity, ige receptor technology, applied in the direction of immunoglobulins, peptides/protein ingredients, peptides against animals/humans, etc., can solve the problems of reduced purification efficiency or production amount in the production of protein pharmaceutical products, poor stability of proteins at low ph, and high cost, and achieve excellent stability and neutralizing activity against ige
a high affinity, ige receptor technology, applied in the direction of immunoglobulins, peptides/protein ingredients, peptides against animals/humans, etc., can solve the problems of reduced purification efficiency or production amount in the production of protein pharmaceutical products, poor stability of proteins at low ph, and high cost, and achieve excellent stability and neutralizing activity against ige
US20180312566A1Inactive Publication Date: 2018-11-01KISSEI PHARMA
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Expression and Preparation of Protein 1
(1) Preparation of Protein 1 Expression Vector
[0068]cDNA encoding the amino acid sequence shown in SEQ ID NO: 3 was incorporated into a mammalian expression plasmid vector to prepare a Protein 1 expression plasmid.
(2) Preparation of Protein 1-Expressing Cells
[0069]The Protein 1 expression plasmid was introduced into Chinese hamster ovary cells (CHO) to establish a Protein 1 stable expression cell line. Secretion of Protein 1 into a culture supernatant was confirmed by SDS-PAGE.
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Provided is an Fc fusion high affinity IgE receptor α-chain having excellent stability at low pH. An Fc fusion protein comprising:(i) a high affinity IgE receptor α-chain; and(ii) an Fc region of IgG1, whereina linker fragment region between the (i) and the (ii) is the amino acid sequence shown in SEQ ID NO: 2.
Description
CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a Continuation of U.S. application Ser. No. 15 / 552,065, which is a National Stage application of PCT / JP2016 / 054854, filed Feb. 19, 2016, which claims priority from Japanese application nos. JP 2015-032231, filed Feb. 20, 2015, and JP 2015-252231, filed Dec. 24, 2015.[0002]The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-WEB and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jul. 10, 2018, is named sequence.txt and is 10 KB.TECHNICAL FIELD[0003]The present invention relates to an Fc fusion high affinity IgE receptor α-chain that is useful as a pharmaceutical product.[0004]More specifically, the present invention relates to an Fc fusion high affinity IgE receptor α-chain having excellent stability at low pH, and a medicinal use thereof.BACKGROUND ART[0005]Immunoglobulin E (IgE) is one of an immunoglobulin group, which plays a role in al...
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Patent Timeline
01 Nov 2018
Publication
US20180312566A1
- IPC
- C07K14/735; C12N15/09; A61K38/16; C07K16/18; C07K14/705; C07K19/00
- CPC
- C07K2319/30; C07K14/70535; C12N15/09; A61K38/16; C07K16/18; C07K14/705; C07K19/00; A61K38/00
- Inventors
- SAKAMOTO, TAKASHI; INADA, YOICHI