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Co-localization at molecular resolution of multiple fluorescence channels acquired using optical microscopy

a fluorescence channel and molecular resolution technology, applied in the field of co-localization at molecular resolution of multiple fluorescence channels acquired using optical microscopy, can solve the problems of lack of morphological information in the overall biochemistry study, and no method to verify whether these biochemical techniques determine real interactions

Inactive Publication Date: 2019-11-07
ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent is about a way to improve the performance of a microscope that uses fluorescence microscopy. It involves using a special setup with multiple fluorescing molecules that can be excited at different wavelengths and have different peak excitation radiation. By using this method, researchers can get better and more accurate imaging of samples.

Problems solved by technology

However, these methods may report adventitious RNA-protein associations that would occur after lysis of cells (12, 13), or functionally important complexes may not survive the procedure.
Importantly, ensemble biochemistry studies lack morphological information, particularly essential for neurons.
Currently there is no method to verify whether these biochemical techniques determine real interactions that take place in the cell.

Method used

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  • Co-localization at molecular resolution of multiple fluorescence channels acquired using optical microscopy
  • Co-localization at molecular resolution of multiple fluorescence channels acquired using optical microscopy
  • Co-localization at molecular resolution of multiple fluorescence channels acquired using optical microscopy

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Embodiment Construction

[0049]A method is provided for improving the performance of a fluorescence microscopy imaging system comprising an optical objective lens, a field of view, an imaging detector, and at least a first and a second fluorescent molecule, each of which fluoresces at a different wavelength than the other and each of which has a different excitation radiation peak than the other fluorescent molecule, the method comprising:[0050]providing in a field of view of the fluorescence microscopy system a plurality of fluorescent beads capable of fluorescing at each of the different wavelengths of the first and second fluorescent molecules, wherein the beads have a diameter lower than a diffraction limit of the optical fluorescence microscopy system;[0051]irradiating the plurality of fluorescent beads at an excitation radiation peak of the first fluorescent molecule and sequentially imaging the fluorescence of each of the plurality of beads within field of view of the fluorescence microscopy system a...

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Abstract

A method for improving the performance of a fluorescence microscopy imaging system and for correcting chromatic aberration of an optical objective in a fluorescence microscopy system.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This application claims benefit of U.S. Provisional Application No. 62 / 451,096, filed Jan. 27, 2017, the contents of which are hereby incorporated by reference.STATEMENT OF GOVERNMENT SUPPORT[0002]This invention was made with government support under grant number NS083085 awarded by the National Institutes of Health. The government has certain rights in the invention.BACKGROUND OF THE INVENTION[0003]Throughout this application various publications are referred to. Full citations for the references may be found at the end of the specification. The disclosures of these publications are hereby incorporated by reference in their entirety into the subject application to more fully describe the art to which the subject invention pertains.[0004]RNA-binding proteins (RBPs) specifically recognize and bind with RNA regulating its life cycle (1, 2). Dysfunctional RNA-protein interaction represents one of causes of genetic disorders that vary from neu...

Claims

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Application Information

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IPC IPC(8): G01N21/64G02B21/02G02B21/16
CPCG02B21/16G01N2021/6441G01N33/582G01N2021/6421G02B21/02G01N2021/6419G01N21/6458G02B21/365
Inventor SINGER, ROBERT H.ELISCOVICH, CAROLINASHENOY, SHAILESH M.
Owner ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIV
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