Multiple Tissue Layer Electroporation Applicator and Device

a multi-tissue layer, electroporation technology, applied in electrotherapy, therapy, etc., can solve the problems of lack of positive data, preventing the successful advancement of electroporation assisted dna vaccine products, etc., and achieve the effect of broad immune respons

Pending Publication Date: 2021-11-18
INOVIO PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The dual depth electroporation device achieves a broad immune response by effectively delivering biomolecules to both muscle and skin tissues, resulting in higher antibody titers and cellular responses compared to single-tissue electroporation methods.

Problems solved by technology

While this technology is known, there have been limitations to it that have prevented successful advancement of electroporation assisted DNA vaccine products.
Still, there has been a lack of positive data shown protective immune response from DNA vaccination to skin tissue along with EP.

Method used

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  • Multiple Tissue Layer Electroporation Applicator and Device
  • Multiple Tissue Layer Electroporation Applicator and Device
  • Multiple Tissue Layer Electroporation Applicator and Device

Examples

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[0046]The present invention is further illustrated in the following Examples. It should be understood that these Examples, while indicating preferred embodiments of the invention, are given by way of illustration only. From the above discussion and these Examples, one skilled in the art can ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. Thus, various modifications of the invention in addition to those shown and described herein will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims.

Animals: Hartley guinea pigs, female, 4 weeks of age with weights between 300 and 350 g, were used in the study (n=5). Animals were housed at Bio-quant, Inc., San Diego, Calif., in accordance with the standards of IACUC.

Plasmids...

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Abstract

The present invention relates to dual depth electroporation devices capable of electorporating both muscle tissue and skin tissue in a single application in order to generate a broad immune response in a subject, and uses of the same.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of U.S. application Ser. No. 13 / 581,700, filed Aug. 29, 2012, which is a National Stage entry of International Application No. PCT / US2011 / 026688; filed Mar. 1, 2011, and claims the benefit of U.S. Provisional Application Nos. 61 / 309,423, filed Mar. 1, 2010, and 61 / 309,860, filed Mar. 2, 2010, the entire contents of which are incorporated herein by reference.BACKGROUND[0002]Electroporation technology is currently being researched in conjunction with DNA vaccines, and other biologicals, to enhance delivery into cells of mammals in vivo. While this technology is known, there have been limitations to it that have prevented successful advancement of electroporation assisted DNA vaccine products. And, as a result, there are been various attempts and modifying or enhancing electroporation technology, whether it relates to the hardware, software, electrical pulse parameter, site of application (on subject), elec...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): A61N1/32
CPCA61N1/327
InventorBRODERICK, KATELIN, FENGMCCOY, JAYKEMMERRER, STEPHEN V.KJEKEN, RUNE
OwnerINOVIO PHARMA