Preparation method and application of subunit vaccine for preventing Staphylococcus-induced mammitis of goats
A technology of subunit vaccine and Staphylococcus, which is applied in the field of subunit vaccine preparation, can solve the problems of short immunity period, large dosage, strong self-toxicity, etc., and achieve the promotion of mucosal immunity, convenient application, and good cross-protection response Effect
Inactive Publication Date: 2013-09-25
NORTHWEST A & F UNIV
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[0003] Traditional mastitis vaccines are mostly live attenuated vaccines or inactivated vaccines. In production practice, they have a certain effect on the prevention and treatment of mastitis. However, with the development of large-scale intensive farming, artificially attenuated strains have homologous Potential possibilities such as recombination and self-virulence recovery, and inactivated vaccines also have shortcomings such as large doses and short immunity periods. In order to improve the safety and immune protection of traditional vaccines, it is extremely important to develop efficient and cheap new vaccines
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[0059] Mastitis subunit vaccine is to use the main protective immunogen of pathogenic bacteria to make a vaccine that does not contain nucleic acid and can induce the body to produce antibodies, and inject it directly into the body to activate the immune system to achieve the purpose of preventing and treating diseases. During the research and development of this vaccine, the selected genes were based on the following fact: ClfA, Hla and Sbi are virulence factors produced by Staphylococcus aureus, and play a virulence role with specific domains in the process of Staphylococcus aureus infecting the host. The A region of ClfA (ClfA-A) is the functional region that binds to provangelin and fibrinogen in the host extracellular matrix; Hla forms a membrane-attacking complex through the interaction between each monomer, and punches holes on the surface of the target cell. Its cleavage, the functional regions of Sbi (I, II, III, IV regions) can specifically bind to the Fc end of the h...
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The invention belongs to the technical field of animal genetic engineering and particularly relates to a method and application of a subunit vaccine for preventing Staphylococcus-induced mammitis of goats. The invention aims to provide the method and the application of the subunit vaccine for preventing Staphylococcus-induced mammitis of goats. The subunit vaccine is similar to the process of natural infection of the pathogenic bacterium, is capable of promoting mucosa immunity, inducing immunologic memory and arousing wide immune response so as to generate an excellent cross protection response; and therefore the subunit vaccine is a novel vaccine which is safer, more stable, is simple to prepare, convenient to use and low in price. The subunit vaccine is prepared by the following steps: 1, expressing and purifying protective antigens of staphylococcus aureus; and 2 mixing recombinant proteins with transfer factor assistants.
Description
1. Technical field: [0001] The invention belongs to the technical field of animal genetic engineering, and in particular relates to a preparation method and application of a subunit vaccine for preventing goat staphylococcus mastitis. 2. Background technology: [0002] Mastitis is the most serious disease in dairy cow and dairy goat farming, often resulting in reduced milk production and poor quality dairy products. Staphylococcus aureus, Escherichia coli, and streptococcus are the main pathogenic bacteria that cause breast infection, clinical and latent mastitis, and there are many side effects of antibiotic treatment for mastitis; [0003] Traditional mastitis vaccines are mostly live attenuated vaccines or inactivated vaccines. In production practice, they have a certain effect on the prevention and treatment of mastitis. However, with the development of large-scale intensive farming, artificially attenuated strains have homologous There are potential possibilities such ...
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IPC IPC(8): A61K39/085A61P31/04C12N15/31C12N15/63C07K14/31
Inventor陈德坤许君艳姚运亮李杰田婷婷罗军
OwnerNORTHWEST A & F UNIV