The invention discloses a preservation device for reproductive embryonic stem cells, and relates to the technical field related to reproductive embryonic stem cells, and the preservation device is characterized by comprising a box body, a display operation screen, a fingerprint identifier, a password input device, a refrigeration box, a liquid nitrogen box, a guide pipe, an electromagnetic valve, a temperature sensor, a servo motor, a rotating shaft, a fixed part and a plurality of brackets, a U-shaped placing piece, a fixed inserting sleeve, a storage pipe, a sealing cover and a rolling piece are arranged at the end part of the bracket; connecting blocks are arranged at the bottoms of the two sides of the fixed inserting sleeve; a guide groove and a spring are arranged on the inner side wall of the U-shaped placing piece; an access hole is formed in the top end of the refrigeration box; a bolt is inserted into the access hole; an opening, a telescopic cylinder, an electronic locking piece, a sealing plate and a lock catch are arranged at the top end of the box body; and a PLC and a power supply device are arranged in the box body. According to the device, the refrigeration effect of other stored embryonic stem cells is not affected when the embryonic stem cells are stored and taken, storing and taking operation is easy and convenient, and meanwhile the device is high in safety and not prone to being stolen.