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Preparing method for cow mammitis staphylococcus culture fluid and its use

A technology for dairy cow mastitis and staphylococcus, which is applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of expensive acceptance, can not truly reflect antibiotic sensitivity, etc., and achieve the effect of inexpensive instruments and equipment

Inactive Publication Date: 2007-10-31
兆丰华生物科技(南京)有限公司
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Problems solved by technology

These methods not only need at least 3-5 days to get the results, but also have the necessary equipment, reagents and technical conditions for bacteriological identification, and the results of drug susceptibility tests with single colonies sometimes cannot truly reflect the sensitivity of the bacterial "population" to antibiotics sex
As for the automated drug susceptibility test method and Etest method, they are difficult to be accepted by domestic veterinary clinics and dairy farms due to their high price.

Method used

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  • Preparing method for cow mammitis staphylococcus culture fluid and its use
  • Preparing method for cow mammitis staphylococcus culture fluid and its use
  • Preparing method for cow mammitis staphylococcus culture fluid and its use

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Embodiment Construction

[0016] 1. Preparation of mBP culture medium:

[0017] Use a balance to weigh 10g of peptone, 5g of yeast powder, 10g of sodium pyruvate, 12g of glycine, and 5g of lithium chloride in a glass container, add 980ml of water, stir evenly and dissolve completely, adjust the pH to 7.3 with sodium hydroxide solution, and Sterilize at 121°C for 15 minutes, cool to 50°C±10°C, add filter-sterilized 1% potassium tellurite solution 10ml and 7ml Tween-80, then store at 4°C for later use.

[0018] Second, the rapid identification of staphylococci:

[0019] According to the clinical examination or the results of the California Mastitis Test (CMT), it is preliminarily judged that the cows have clinical or latent mastitis. The first three handfuls of milk are squeezed out by conventional milking methods, and the teats and their surrounding areas are disinfected with conventional disinfectants. Cotton balls to sanitize nipples. Open the cover of the sterilized test tube, keep the test tube ho...

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Abstract

The preparation process of cow mammitis staphylococcus culture fluid and its use belongs to the field of animal epidemic disease diagnosing, preventing and treating technology. The preparation process includes dissolving peptone 10 g, yeast powder 5 g, sodium pyruvate 10 g, glycin 12 g and lithium chloride 5 g in water of 980 ml; regulating pH to 7.3 with sodium hydroxide; sterilizing at 121 deg.c; cooling to 50+ / -10 deg.c; adding bacteria-free 1% concentration potassium tellurite water solution in 10 ml and Tween-80 in 7 ml, and preservation at 4 deg. c. The culture fluid may be used in the fast identification of cow mammitis staphylococcus and antibiotic sensitivity test.

Description

technical field [0001] The invention belongs to the technical field of animal epidemic diagnosis and prevention. technical background [0002] Dairy cow mastitis has a high incidence and great harm, and is one of the most important diseases affecting the development of the dairy industry in the world. According to the International Milk Federation, about 50% of cows suffer from mastitis, of which about 2% suffer from clinical mastitis and 48% suffer from recessive mastitis. The incidence rate of clinical mastitis in my country is 33.41% (9.7%-55.6%), and the detection rate of recessive mastitis is 50-80%. Clinical mastitis has obvious clinical symptoms such as decreased milk production, breast swelling, and milk deterioration, while subclinical mastitis has no visible changes in the appearance of breasts and milk, but often causes the quality of raw milk to decline, threatening people's health. healthy. It is estimated that the annual economic loss caused by mastitis in m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04
Inventor 孙怀昌仇华磊钱科张鑫宇
Owner 兆丰华生物科技(南京)有限公司
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