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Highactive wild Chinese caterpillar fungus strain, and its fruiting body and artificial culture method

A technology of artificial cultivation and wild Cordyceps, applied in the field of microorganisms, can solve problems such as rising prices of Cordyceps sinensis, improve the quality of life and health level, enrich the nutritional value and health care effects, and alleviate the shortage of Cordyceps resources.

Active Publication Date: 2008-05-21
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, Cordyceps sinensis has not been cultivated on a large scale, and the vicious circle has caused the price of Cordyceps to rise year by year, far from meeting people's growing demand.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] Utilize the high-activity wild Cordyceps new bacterial strain of the present invention, i.e. Japanese Cordyceps japanese variant GDIM_C050423 CCTCC M206051 bacterial strain cultivation to produce the process of high-activity wild Cordyceps fruiting body as follows:

[0027] 1. Strain isolation, preservation and rejuvenation

[0028] 1.1 Original strain

[0029] The original strain was the GDIM_C050423 CCTCC M 206051 strain of Cordyceps japonicus Guangdong variant found in the wild in Guangdong and obtained by tissue isolation.

[0030] 1.2 Mother species

[0031] Insert the original strains into the aseptic enriched PDA slant medium, culture in the dark for 10-15 days at 20-25°C, and then light for 4-6 days, select the strains with fast color change and neat hyphae growth, as mother species.

[0032] 1.3 Rejuvenation

[0033] After several times of cultivation, the mother species with good quality is selected, and 1-2% glucose is added to the culture medium for reju...

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Abstract

The invention supplies a high activity wild Chinese caterpillar fungus strain and the artificial planting method. The Chinese caterpillar fungus strain is GDIM_C050423 CCTCC M 206051 that could produce fruiting body with high activity material. The method includes mother strain making, first level liquid strain making, second level liquid strain making, inoculating, artificial plating and gathering. The fruiting body has the feature of rod shaped, no branch or only has short branches at the top, and tip shaped top. It has abundant nutritive value and health protection function. It contains over 39.2% cordycepic acid.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to highly active wild Cordyceps strains, their fruit bodies and an artificial cultivation method. Background technique [0002] The genus Cordyceps belongs to the phylum Ascomycota, the class Ascomycota, the order Hypocreales, and the family Clavcipitaceae. Fungi of the genus Cordyceps have a long history of medicinal use in my country, especially the most famous Cordyceps sinensis, which has a variety of therapeutic and nourishing health effects, and is well-known at home and abroad. In addition, Cordyceps militaris (also known as Cordyceps militaris) (C.militaris.), cicadae (C.sobolifer), Gunni Cordyceps (C.gunnii.) etc. all have the effect similar to Cordyceps sinensis, some species have more active ingredients than Cordyceps sinensis To be tall (Chen Wanqun et al., 1993). [0003] Modern scientific research shows that various active ingredients such as cordycepin, cordycepic ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 李泰辉林群英宋斌钟月金沈亚恒吴丽明黄浩
Owner GUANGDONG INST OF MICROORGANISM