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Method of simultaneously separating and cultivating pathogenicity Neisseria and mycoplasma

A simultaneous separation and pathogenicity technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of complicated operation and high cost, and achieve the effect of simple operation and saving usage

Inactive Publication Date: 2008-12-31
上海恩康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This traditional method of isolating and cultivating suspicious pathogenic microorganisms has defects such as complicated operation and high cost, and needs to be improved and improved to ensure the specificity, sensitivity and repeat stability of clinical diagnosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for simultaneously isolating and cultivating pathogenic Neisseria and mycoplasma, the method comprising the following steps:

[0017] (1) Inoculation, firstly take the material from the suspicious patient specimen, and then directly inoculate it on the culture medium of the inner chip of the sealed biological culture component;

[0018] (2) Cultivate, place CO in the module 2 Generator sheet, micro-release oxygen generation sheet and cover with airtight cap, incubate at 30°C for 72 hours;

[0019] (3) Observing and separating, pathogenic Neisseria can grow needle-like transparent colonies visible to the naked eye, and pathogenic Mycoplasma can be observed as brown colonies under a 40-100 times microscope.

[0020] The airtight biological culture component is composed of a transparent outer sleeve, a sealing cover matched with the outer sleeve, and an inner chip, and the outer sleeve is flat.

[0021] The weight formula of described culture medium and preparat...

Embodiment 2

[0023] A method for simultaneously isolating and cultivating pathogenic Neisseria and mycoplasma, the method comprising the following steps:

[0024] (1) Inoculation, firstly take the material from the suspicious patient specimen, and then directly inoculate it on the culture medium of the inner chip of the sealed biological culture component;

[0025] (2) Cultivate, place CO in the module 2 Generator slices and anaerobic generator slices were covered with airtight lids and incubated at 40°C for 24 hours;

[0026] (3) Observing and separating, pathogenic Neisseria can grow needle-like transparent colonies visible to the naked eye, and pathogenic Mycoplasma can be observed as brown colonies under a 40-100 times microscope.

[0027] The airtight biological culture component is composed of a transparent outer sleeve, a sealing cover matched with the outer sleeve, and an inner chip, and the outer sleeve is flat.

[0028] The weight formula of described culture medium and prepara...

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Abstract

The invention relates to a method for simultaneously isolating and cultivating pathogenic Neisseria and mycoplasma, the method comprising the following steps: (1) inoculating, first taking materials from suspicious patient specimens, and then directly inoculating them in the sealed biological culture assembly on the culture medium of the chip; (2) culture, place a gas generating sheet in the assembly and cover the airtight cover, and cultivate at 30-40°C for 24-72 hours; (3) observe the separation, the pathogenic Neisseria can Growth of needle-shaped transparent colonies visible to the naked eye, and pathogenic mycoplasma can be observed under a 40-100 times microscope as brown colonies. Compared with the prior art, the present invention has the advantages of simple operation and low cost.

Description

technical field [0001] The invention relates to a method for simultaneously isolating and cultivating pathogenic Neisseria and mycoplasma, which can be used in clinical medicine to isolate and cultivate pathogenic Neisseria (Neuisseria gonorrhoeae and meningitidis) cocci) and mycoplasma (Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma pneumoniae). Background technique [0002] Humans are infected by many pathogenic microorganisms and suffer from infectious diseases. In clinical medicine, it is necessary to carry out clear pathogen analysis and testing on patients with suspected infection to help doctors diagnose and treat diseases. The laboratory test for microbial isolation of infectious disease pathogens must rely on the isolation of pathogenic microorganisms from suspicious patient physical examination specimens to determine the type of pathogenic microorganisms to help doctors choose which antibacterial agents to treat. Conventional or traditional means and m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/35C12R1/36
Inventor 叶元康
Owner 上海恩康生物科技有限公司