Preparation method of gelling agent for culture medium carrier

A gel and culture medium technology, applied in the field of polymer materials, can solve the problems that grassroots units and food companies cannot implement on-site inspections and take a long time

Inactive Publication Date: 2009-02-25
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Third, the conventional method usually takes a long time to prepare the culture medium and utensils for testing, which makes it imposs

Method used

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Examples

Experimental program
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Embodiment Construction

[0034] One, the preparation method 1 of the gel agent that is used for culture medium carrier:

[0035] (1) prepare the sodium hydroxide solution that final concentration is 30% (g / g) with deionized water;

[0036] (2) Add the sodium hydroxide solution dropwise to 18.53 mL of acrylic acid stirred by a magnetic stirrer at a constant temperature (25° C.) with a basic burette to achieve a neutralization degree of 75%;

[0037] (3) Add 1.16 mL of deionized water to adjust the concentration of acrylic acid monomer to 50% (g / g), and then transfer the mixture to a plastic three-necked bottle;

[0038] (4) After adding the crosslinking agent N, N-methylenebisacrylamide and mixing, add 0.0271g, which is 0.1% (g / g) of the acrylic acid monomer;

[0039] (5) Add initiator ammonium persulfate under the situation of nitrogen blowing, the addition is 0.5% (g / g) of acrylic acid monomer, i.e. 0.1355g;

[0040] (6) Blow nitrogen to drive oxygen to seal, and place in a constant temperature dry...

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Abstract

The invention discloses a making method of gelling agent of culture medium carrier in the macromolecular material domain, which comprises the following steps: grinding raw materials with sodium polyacrylate, xanthan gum and locust bean gum; sieving over 160-order; blending grinded xanthan gum and locust bean gum with molar rate at 3:2-5 evenly; blending the composite and grinding sodium polyacrylate with molar rate at 5:0.8-2.0 evenly to obtain the gelling agent.

Description

【Technical field】 [0001] The invention relates to a preparation method of a gel used for a culture medium carrier, belonging to the field of polymer materials. 【Background technique】 [0002] Microbiological rapid test card refers to the use of paper, paper film, film, etc. as the medium carrier, on which specific medium and chromogenic substances are adhered to it through a specific gel, and determined by the growth and coloration of microorganisms on it. Methods of Microorganisms in Food. In 1955, German scholar FJ. Forg invented a simple and fast rapid detection method for coliform bacteria - the paper method, which shortened the original detection cycle from 72 hours to 15 hours, reduced the cost of materials by 3 / 4, and greatly Simplified operating procedures. Since then, this detection method integrating chemistry, macromolecular science and microbiology began to develop. Rapid assay card detection has significant advantages: first, it can measure a small amount of ...

Claims

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Application Information

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IPC IPC(8): C08L33/02C08J3/00C12Q1/04C08L5/00
Inventor 吴清平孙永蔡芷荷郭伟鹏
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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