Uses of spatial configuration to modulate protein function

A technology of interferon and compound interferon, which is applied in the direction of medical preparations containing active ingredients, nebulizers for treatment, peptide/protein components, etc., can solve the problem of not being able to inhibit e-antigen and s-antigen

Active Publication Date: 2007-07-18
SUPERLAB FAR EAST LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All interferons can only inhibit viral DNA replication, but not e antigen and s antigen

Method used

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  • Uses of spatial configuration to modulate protein function
  • Uses of spatial configuration to modulate protein function
  • Uses of spatial configuration to modulate protein function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0152] Recombinant high-efficiency composite interferon (rSIFN-co) is a new interferon molecule constructed by genetic engineering from the most common conserved amino acids in several natural human alpha interferon subtypes. It has been proved that rSIFN-co has broad-spectrum interferon activity, such as strong antiviral and tumor suppressive activity, especially in anti-chronic hepatitis C.

[0153] According to the published rSIFN-co coding DNA sequence and deduced amino acid sequence (Fig. 1), we redesigned the cDNA coding sequence of rSIFN-co using the preferentially expressed codons in Escherichia coli, and then artificially synthesized the full-length cDNA gene of rSIFN-co.

[0154] In order to obtain high-purity rSIFN-co protein, the full-length cDNA sequence of rSIFN-co was cloned into the high-efficiency expression vector of Escherichia coli, and then the strong P in the vector was induced and activated by L-arabinose BAD The promoter mediated the high expression of ...

Embodiment 2

[0263] Isolation and purification of rSIFN-co

[0264] 1. Fermentation

[0265] Inoculate the recombinant strains in LB medium and culture overnight (about 18 hours) at 37°C in shake flasks (200rpm), add 30% glycerol to the fermentation broth to a final concentration of 15%, divide into 1ml each, and store at -20°C Preserved as a production strain.

[0266] The production strain was added to LB medium at a ratio of 1%, and the scale of the strain was expanded overnight by culturing in a shake flask (200 rpm) at 37°C. Add 10% into RM medium and culture at 37°C. Ferment to OD 600 To reach 2.0 or so, add arabinose (20%) to a final concentration of 0.02% for induction. Stop culturing after 4 hours, centrifuge, and collect bacteria. The bacterial cell pellet was resuspended with an appropriate amount of buffer A, placed at -20°C to pass through the liquid, taken out and melted, destructed with a homogenizer, centrifuged, washed with buffers B and C separately, and washed once ...

Embodiment 3

[0331] Stability of recombinant high-efficiency compound interferon freeze-dried powder for injection

[0332] We conducted a stability test on three batches of samples of the recombinant high-efficiency compound interferon freeze-dried powder, each with two specifications. The test start time: April 2000.

[0333] 1. Sample source

[0334] The samples were provided by Sichuan Huiyang Life Engineering Co., Ltd., and the batch numbers are: 990101-03, 990101-05, 990102-03, 990102-05, 990103-03, 990103-05.

[0335] 2. Sample standard

[0336] Each sample in this experiment should meet the requirements in the table below before the test.

[0337] Table 1 Test sample standard

[0338] indicators

standard

1. Appearance

white puffy body

2. Dissolving time

Water for injection dissolves rapidly at room temperature (within 2 minutes)

3. Clarity

Colorless or slightly opalescent clear liquid, should not be turbid, contain foreign mat...

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Abstract

This invention provides a method for preventing or treating Severe Acute Respiratory Syndrome in a subject comprising administering to the subject an effective amount of recombinant super-compound interferon or a functional equivalent thereof. This invention provides a method for inhibiting the causative agent of Severe Acute Respiratory Syndrome comprising contacting the agent with an effective amount of super-compound interferon or its equivalent.

Description

[0001] This application claims U.S. Patent Application No. 60 / 498,449, filed August 28, 2003, U.S. Patent Application No. 60 / 498,785, filed August 28, 2003, and U.S. Patent Application No. 60 / 498,785, filed August 28, 2003. Priority of Patent Application No. 60 / 498,923. This application also claims priority from Indian Patent Application No. 279 / MUM / 2004 filed on March 5, 2004 and Indian Patent Application No. 280 / MUM / 2004 filed on March 5, 2004. The content of the above application is incorporated by reference in its entirety into this application. [0002] Various references are cited throughout this application. These publications in their entirety are incorporated into this application by reference which, in connection with this invention, are intended to fully describe the state of the art to which this invention pertains. technical field [0003] The invention relates to a recombinant super-compound interferon (rSIFN-co) with changed spatial conformation. One of the c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/21A61M11/00C12N15/11
Inventor 魏光文
Owner SUPERLAB FAR EAST LTD
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