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Device for the detection of fluorescence emitted by chromophoric elements in the wells of a multiwell plate

A technology of chromophores and porous plates, which is applied in the direction of fluorescence/phosphorescence, laboratory containers, measuring devices, etc., can solve the problems of expensive and achieve rapid acquisition results

Inactive Publication Date: 2007-07-18
热内瓦韦公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The scanning confocal microscopy technique can effectively separate markers of cells or molecules immobilized on the bottom of the well from those suspended in the liquid contained within the well, however, this method is expensive and very slow

Method used

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  • Device for the detection of fluorescence emitted by chromophoric elements in the wells of a multiwell plate
  • Device for the detection of fluorescence emitted by chromophoric elements in the wells of a multiwell plate
  • Device for the detection of fluorescence emitted by chromophoric elements in the wells of a multiwell plate

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Embodiment Construction

[0036] Figures 1 and 2 are diagrams of a standardized type of multiwell plate 10 used very widely in biology and pharmacy, said plate 10 being made of molded plastic material and having a very large number of wells 12 arranged in an array, The holes are closed at their bottom ends by mating plates 14 of transparent material, such as plastic material, glass or quartz.

[0037] In a variant, the plate 10 may be molded in a single piece of transparent plastic material.

[0038] In the example shown, the plate 10 has 96 holes, typically having an inner diameter in the range of 5 mm to 8 mm, a depth in the range of 1 mm to 10 mm, and spaced apart from each other by a distance equal to 2.25 mm, depending on the implementation. Or 4.5 mm or 9 mm, the hole 12 is cylindrical or slightly tapered, as schematically shown in FIG. 2 .

[0039] These standardized plates are operated by a robot that puts a predetermined amount of liquid (sample, reagent, wash and rinse, etc.) into wells 12 to ...

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Abstract

The invention relates to a device for the detection of fluorescence emitted by chromophoric elements contained in the wells of a multiwell plate. The inventive device comprises means which are built into the transparent bases of the wells (12) of the plate (10) in order to limit the well penetration depth of a light beam used to excite the chromophoric elements (38) which are fixed to the bases (14) of the wells.

Description

technical field [0001] The present invention relates to a device for detecting fluorescence emitted by chromophore elements contained in wells of a multiwellplate of the type used in biology and pharmacy . Background technique [0002] These standard plates have a very large number of wells arranged in an array, and they can be handled by a robot, which puts into them quantified liquids for the reactions involved in sample detection, DNA hybridization, and the like. [0003] Cells or molecules of biological interest housed in these wells can be specifically labeled by chromophore moieties that emit light of one narrow wavelength band in response to excitation of another narrow band of wavelengths. Fluorescence in the wavelength band, the role of the emitted fluorescence is to display the marked cells or molecules or some of their characteristics. For example, antibodies can be detected on the surface of cells selected on the basis of the amount of fluorescent marker they h...

Claims

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Application Information

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IPC IPC(8): G01N21/77B01L3/00G01N21/64
CPCG01N2021/6484G01N21/6452G01N2201/06193G01N2201/0826
Inventor 克洛德·韦斯比什马克西姆·拉捷乔治·奥利维尔·雷蒙德乌塔伊·舒马内
Owner 热内瓦韦公司